Page:Bergey's manual of determinative bacteriology.djvu/775

 A. Limited proteolytic action in gelatin, milk, coagulated egg-albumin or fibrin. 1. Vegetative growth white.

a$1$. No soluble pigment in organic media.

141. Streptomyces listeri.

a$2$. Soluble pigment in organic media brown to brown-red.

142. Streptomyces galtieri. 2. Vegetative growth cream-colored; aerial mycelium scant, white.

143. Streptomyces upcotlii. 3. Growth very limited on various media, except on potato plug; no liquefaction of gelatin.

144. Streptomyces hortonensis. B. Strong proteolyetic action in gelatin and milk.

1. Growth on potato plug moist, membranous.

a$1$ Pigment deep brown.

145. Streptomyces beddardii.

a$2$. Pigment faint brown.

146. Streptomyces kimberi.

2. Growth on potato plug abiuidant, becoming black; aerial mycelium white-gray; plug discolored.

147. Streptomyces somaliensis. 3. Growth on some media pink.

148. Streptomyces panjae.

4. Aerial mycelium on most media profuse, white; spiral formation.

149. Streptomyces willmorei. V. No aerial mycelium.

150. Sterile (non-conidia-forming) species.

1. Streptomyces albus (Rossi-Doria, 1891, emend. Krainsky, 1914) Waksman and Henrici, 1943. (Streptotrix (sic) alba Rossi- Doria, Ann. d'Ist. d'Ig. sper. d. Univ. di Roma, 1, 1891, 399; Actinomyces albus Krainsky, Cent. f. Bakt., II Abt., 41, 1914, 662; Waksman and Henrici, Jour. Bact., 46, 1943, 339.)

al'bus. L. adj. albus white.

The description of this species by Rossi- Doria is incomplete. The characters given below are taken from Krainsky (op. cit., 1914, 662) with some supplementary infor- mation from later authors. Other descrip- tions which may vary from this in certain details are given by Waksman and Curtis (Soil Sci., 1, 1916, 117), Bergey et al. (Man- ual, 1st ed., 1923, 367), Duché (Les actino- mycesdugroupe albus, Paris, 1934, 257) and Baldacci (Mycopathologia, 2, 1940, 156).

Vegetative growth: Hyphae branched, 1 micron in diameter.

Aerial mycelium: Abundant, white. Hyphae 1.3 to 1.7 microns in diameter with ellipsoidal spores (1 micron long) in coiled chains on lateral branches of the aeria hyphae.

Gelatin: Rapid liquefaction. Gray colonies. No soluble pigment.

Agar: No aerial mycelium, but a chalky white deposit forms on old colonies.

Ca-malate agar: Colonies of medium size; he center only is covered with a white aerial mycelium.

Starch agar: White aerial mycelium covering the whole surface.

Glucose agar: Gray aerial mycelium becoming brownish.

Broth: Flaky growth on bottom with cultures. White aerial mycelium.

Milk: Rapidly peptonized after coagulation. Reaction becomes alkaline (Duché). Cream-coloured surface ring. White arial mycelium.

Potato: White aerial mycelium. Growth folded, cream-colored.

Carrots and other vegetables: Excellent growth (Duché).

No hydrolysis of starch in some cultures; rapid hydrolysis in others.