Page:Bergey's manual of determinative bacteriology.djvu/759

 opaque reddish purple after 2 months; hy- drolyzed, clear wine-red after 3 months. Antagonistic properties: Positive. Source : Isolated from a case of mycetoma of a foot in South Africa. Habitat: Unknown. 38. Nocardia pelletieri (Laveran, 1906) Pinoy, 1912. (Micrococcus pelleiieri Lixvemn, Compt. rend. Soc. Biol., Paris, 61, 1906, 340; Oospora pelletieri Thirou.x and Pelletier, Bull. Soc. path, exot., 5, 1912, 588; Pinoy, in Thiroux and Pelletier, ibid., 589; Strep- tomyces pelletieri Waksman and Henrici, in Manual, 6th ed., 1948, 960.) pel.le.ti.e'ri. M.L. gen. noun pelletieri of Pelletier; named for M. Pelletier, the first to isolate this species. Description taken from Erikson (Med. Res. Council Spec. Rept. Ser. 203, 1935, 21). Mycelium composed of slender, straight and not very long filaments, forming small, dense, pink colonies with a few short, straight, isolated aerial branches. Gelatin: Slight liquefaction; few pink flakes; later almost completely liquefied. Agar: Minute, colorless colonies and piled-up, pale pink masses. Glucose agar: Poor growth; a few minute pink colonies. Glj^cerol agar: Poor growth; a few moist pink colonies. Ca-agar: Colorless, small colonies; after 1 week, confluent skin, pink, buckled; me- dium discolored later. Coon's agar: Poor growth, cream-colored with pink center, mostly submerged. Potato agar: Colorless blister colonies; after 3 weeks, colonies larger, showing con- centric zones, submerged margins and oc- casional zone or tuft of white aerial my- celium, pinkish coloration. Dorset's egg medium: Abundant, wrin- kled, pink skin with small discrete colonies at margin in six days; later surface rough, mealy; considerable liquefaction in 17 days. Serum agar: Moist, cream-colored growth tending to be heaped up; discrete colonies at margin; becoming umbilicated. Inspissated serum: Round, moist, color- less colonies. Blood agar: At first a few pinhead, cream- colored colonies, no hemolysis; later col- onies dense, button-shaped, with narrow fringed margin. Broth: Small, minute, pink, clustered colonies. Synthetic sucrose solution: Small, pink colonies in sediment; later minute colonies adhering to side of tube. Milk: Soft curd; half -digested; peptoniza- tion complete in 20 days. Litmus milk: Pink surface growth, semi- solid, no color change; after 20 days, coagu- lum cleared, liquid purple. Potato plug: After one month growth sparse, yellowish pink, irregularly piled up, portions with scant white aerial mycelium; after 6 months abundant, highly piled-up, small, rounded pink masses; scant white aerial mycelium persistent. Relationships to other species: Thiroux and Pelletier (Bull. Soc. path, exot., 5, 1912, 585) considered that their cultures resem- bled Nocardia madtirae, but they grew the organism only on Sabouraud's gelatin, on which it appeared in a constantly red, easily detachable form. Nocardia indica was re- garded as identical by Pinoy, although in the original description by Laveran the organism was called Micrococcus pelletieri, owing to the fact that no mycelium was seen, merely coccoid bodies. Nocardia genesii Froes (Bull. Inst. Past., 29, 1931, 1158) is described as closely allied, the dis- tinction being founded upon the fact that the red grains were smaller in size and much more numerous, but no cultural details are given. Source: Isolated from a case of crimson- grained mycetoma in Nigeria (E. C. Smith, Trans. R. Soc. Trop. Med. Hyg., 22, 1928, 157). Habitat: L^nkiiown. 39. Nocardia niaculata (Millard and Burr, 1926) Waksman and Henrici, 1948. {Actinomyces maculatus Millard and Burr, Ann. Appl. Biol., 13, 1936, 580; Proactino- myces maculatus Umbreit, Jour. Bact., 38, 1939, 84; Waksman and Henrici, in Manual, 6th ed., 1948, 913.) ma.cu.Ia'ta. L. i)art.adj. tnaculatus spotted. Filamentous organisms possessing a ough, shiny colony which is cartilaginous.