Page:Bergey's manual of determinative bacteriology.djvu/750

 Dorset's egg medium: Raised, smooth, moist, verrucose, buff -colored growth. Loeffler's medium: After 10 daj^s, slight growth, dry, granular, pale buff-colored. Broth: Turbid. Litmus milk: Slightly alkaline after 5 to 7 days. Glycerol potato: After 2 days, dry, crumpled, orange, becoming brown after about 10 da3^s. No acid from glucose, lactose, sucrose or glycerol. Phenol is utilized. Nitrites not produced from nitrates. Optimum temperature, between 22° and 25° C. Optimum pH, between 7.6 and 8.0. Distinctive characters: Differs from the previously described members of the genus in the absence of chromogenesis. Forms hollow lobes in deep gelatin cultures. Cells are rods, seldom filaments. Source : Isolated from soil in Great Britain and Australia. Habitat: Presumably soil. 19. Nocardia transvalensis Pijper and Pullinger, 1927. (Jour. Trop. Med. Hyg., 30, 1927, 153.) trans. va.len'sis. M.L. adj. transvalensis pertaining to the Transvaal; named for Transvaal, South Africa. Description taken from Erikson (Med. Res. Council Spec. Rept. Ser. 203, 1935, 28). Initial mycelium unicellular, but with the central branch frequently broader and showing dense, granular refractile contents; small colonies quickly covered with aerial mycelium, the straight aerial hyphae in some cases becoming clustered into irregular spikes; colorless drops are exuded and a pink coloration produced in the densest part of the growth on synthetic glycerol agar. Angular branching with division of the sub- stratum filaments can be seen, the aerial hyphae also being irregularly segmented. Acid-fast. Gelatin: Poor growth; there are a few irregular colorless flakes. No liquefaction. Agar: No growth. Glucose agar: Raised, granular, pink colonies with white aerial mycelium. Glycerol agar: Small, pink, coiled masses with thin, white, aerial mycelium. Potato agar: No growth. Coon's agar: Colorless growth with liberal, white, aerial mycelium. Dorset's egg medium: Small, irregularly raised and coiled dull pink mass. Serum agar: Very poor growth. Inspissated serum: Scant, colorless, flaky growth; later a minute tuft of pale pink, aerial mycelium. Broth: Moderate, flaky growth. Synthetic sucrose solution : Poor growth, a few flakes on the surface and a few at the bottom. Milk: No change. Potato plug: Dry, raised, convoluted, pink growth with white aerial mycelium in one month; dull, pink, brittle surface colonies with paler aerial mycelium float- ing coherently on liquid at base in 2 months. Starch not hydrolyzed. Source: Isolated from a case of mj^ce- toma of the foot in South Africa. Habitat: Found in human infections so far as known. 20. Nocardia mesenterica (Orla-Jen- sen, 1919) Waksman and Henrici, 1948. (Microbacterium mesentericum Orla-Jensen, The Lactic Acid Bacteria, 1919, 181; Pro- actinormjces mesentericus Jensen, Proc. Lin- nean Soc. New So. Wales, 57, 1932, 373; Waksman and Henrici, in Manual, 6th ed., 1948, 907.) me.sen.te'ri.ca. Gr.noun mesenterium the mesentery; M.L. adj. mesentericus per- taining to the mesentery. Extensive mycelium composed of richly branching hyphae of a somewhat variable diameter, 0.4 to 0.8 micron; no aerial hyphae are seen. With increasing age the hyphae divide into fragments of varjang size and shape, partly diphtheroid rods, but no real cocci. There is, particularly in richer media, a tendency to form large, swollen, fusiform to almost spherical cells, up to 3.5 microns in diameter. These may stain intensely with carbol fuchsin; when transferred to fresh media, they germinate and produce a new mycelium. Gelatin: Good growth; finely arborescent, cream-colored growth in the stab; raised,