Page:Bergey's manual of determinative bacteriology.djvu/745

 Source: Original isolate, BG 19, came from a bud-proliferating gall on a blueberry plant. Habitat: Found on blueberry plants so far as known. 10. Nocardia piilnionalis (Burnett, 1910) Waksman and Henrici, 1948. {Actino- myces pulmonalis Burnett, Ann. Rept. N.Y State Vet. Coll., 1909-1910, 167; Waksman and Henrici, in Manual, 6th ed., 1948, 901.) pul.mo.na'lis. L. noun pulmo the lung; L. gen. noun pulmonis of the lung; M.L. adj. pulmonalis pertaining to the lung. Gram-positive mycelium breaking up readily into oval-shaped conidia. Acid-fast, especially in early stages of growth. Gelatin: Small, whitish, spherical col- onies, edges of colony becoming chalky white. Limited liquefaction. Agar: Moist, raised growth in the form of small, spherical colonies. Glucose agar: Dull, whitish, convoluted growth. Broth: Delicate, translucent film on sur- face, becoming corrugated with some whit- ish, spherical colonies in medium. Milk: Colonies on the surface of the me- dium; milk is coagulated in a few days, later digested. Potato: Luxuriant growth in the form of small, translucent, round colonies which become lemon-yellow; later, growth be- comes convoluted or folded with chalky white aerial mycelium, color of plug brown- ish. Non-pathogenic for rabbits or guinea pigs. Aerobic. Source: Isolated from the lungs of a cow. Habitat: Found in bovine infections so far as known. 11. Nocardia paraffinae (Jensen, 1931) Waksman and Henrici, 1948. {Proactino- myces paraffinae Jensen, Proc. Linn. Soc. New So. Wales, 66, 1931, 362; Waksman and Henrici, in Manual, 6th ed., 1948, 901; also see Erikson, Jour. Gen. Microbiol., S, 1949, 366.) pa. raf.fi 'nae. M.L. noun paraffina paraf- fin; M.L. gen. noun paraffinae of paraffin. In agar media the organism initially forms an extensive mycelium of long, richly- branching hyphae, 0.4 to 0.5 micron in diameter. After 5 to 6 days at room tempera- ture, numerous end-branches swell to about double thickness, become more refractive, exhibit fine incisions along their external contours and divide into ovoid, spore-like elements, 0.8 to 1.0 by 1.2 to 1.5 microns. This process of division starts at the tips of the swollen branches and proceeds basi- petally until most of the hyphae appear di- vided. Primary septa have not been seen in the hyphae. A similar process of division takes place in liquid media, where also the filaments often fall into fragments of vari- able length. The spore-like elements, but not the undivided filaments, are markedly acid-fast. Acid-fastness is observed in cer- tain stages, notably in the early stages of growth and in the coccus forms (Erikson, loc. cit.). The aerial mycelium consists of rather short, straight, not very much branched hyphae, 0.4 to 0.6 micron in di- ameter, which never show any differentia- tion into spores. Gelatin: No liquefaction. Sucrose agar: Very scant growth. Thin, colorless veil, sometimes with a trace of white aerial mycelium. Glucose agar: Fair growth. Vegetative mycelium flat, growing into medium; pale ochre-yellow to orange, w'ith raised out- growths on the surface. Growth of a crumbly consistency. Scant, white, aerial mycelium. Nutrient agar: Slow but good growth. Vegetative mycelium superficial, somewhat raised, ochre-yellow, hard, but with a loose, smeary surface. Aerial mycelium scant, small white tufts. No pigment. Liquid media (milk, broth, synthetic solu- tions) : Small, round granules of various yellow to orange colors, firm, but can be crushed into a homogeneous smear. In old broth cultures a thick, hard, orange to brownish surface pellicle is formed. Milk: No coagulation or digestion. Potato: Fair growth. Vegetative myce- lium granulated, first pale-yellow, later deep ochre-yellow to orange. Scant, white, aerial mycelium. No diffusible pigment. Sucrose not inverted. Starch not hydrolyzed. Cellulose not decomposed.