Page:Bergey's manual of determinative bacteriology.djvu/744

 Habitat: Found in infections of goats so far as known. 8. Nocardia pretoriana Pijper and Pul- linger, 1927. (Jour. Trop. Med. and Hyg., 30, 1927, 153.) pre.to.ri.a'na. M.L. adj. pretorianus pertaining to Pretoria; named for Pretoria, South Africa. Description taken from Erikson (Med. Res. Council Spec. Rept. Ser. 203, 1935, 30). Minute, fiat colonies are formed consisting of angularly branched filaments and bearing a few short, straight aerial hyphae; later the growth becomes spreading and extensive, the slipping of the branches is well marked and the aerial hyphae are divided into cylindrical conidia. Slightly acid-fast. Gelatin: A few, colorless flakes. No lique- faction. Glucose agar: Pale buff, umbilicated and piled up colonies. Glycerol agar: Piled up pink mass; very scant, white aerial mycelium at margin. Ca-agar: Yellowish, wrinkled, coherent growth with white aerial mycelium on apices and at margin. Coon's agar: Colorless, mostly submerged growth; scant white aerial mycelium. Dorset's egg medium: A few, round, color- less colonies in 3 days; after 3 weeks, irregu- lar, raised, pink mass; warted appearance; moderate degree of liquefaction. Serum agar: Raised, convoluted, slightly pinkish growth. Inspissated serum: No growth. Broth: Moderate quantity of flakes and dust-like surface growth. Synthetic sucrose solution: A few color- less flakes on the surface; lesser bottom growth. Milk: Yellowish surface growth; solid coagulum in one month; later, partly di- gested, pale pink growth up the wall of the tube. Litmus milk: Colorless surface growth; liquid blue; becomes hydrolyzed and de- colorized. Potato plug: Small, raised, pale pink colonies with white aerial mycelium; after 2 months, plug and liquid discolored, growth dull buff, dry and convoluted at base, round and zonate at top of slant, white aerial mycelium, surface and bottom growth on liquid. Source : Isolated from a case of mycetoma of the chest wall in a South African native. Habitat: Found in human infections so far as known. 9. Nocardia vaccinii Demaree and Smith, 1952. {Actinomyces sp. Demaree, Phytopath., 37, 1947, 438; Demaree and Smith, Phytopath., 4^, 1952, 249.) vac.cin'i.i. M.L. noun Vaccinium generic name of the blueberry; M.L. gen. noun vaccinii of Vaccinium. Rods and filaments, 0.4 to 0.8 micron in diameter, granular when stained, eventually breaking up into bacillary forms. A few cells are acid-fast. Presence of fat was demon- strated by staining with Sudan black B. Gelatin: Dry, ribbon growth; no hydroly- sis. Agar: Poor, slow, granular, gray growth which is sometimes pinkish in old cultures. Synthetic agar: Scant, gray growth. Starch agar: Dry, ribbon, pinkish to orange growth. Potato-yeast-mannitol agar: Abundant, fluffy, gray to orange growth. Milk: Dry, raised, gray growth with orange spots. Casein not hydrolyzed. Potato: Slow, spreading, raised, gray growth. On a basal agar with ammonia as a source of nitrogen, acid was produced from glu- cose, sucrose, glycerol and mannitol; reac- tions variable with arabinose and xylose; no growth on lactose or sorbitol. Starch is hydrolyzed. Citrates utilized to a limited extent. Paraffin is utilized. Nitrites slowly produced from nitrates. Optimum temperature, between 25° and 28° C; inhibited at 32° C; no growth or very scant growth at 37° C. Antagonistic properties : None. Distinctive characters: Resembles No- cardia 7ninima but diff'ers from it in the following respects: utilizes glycerol and mannitol and sometimes arabinose and xylose; reduces nitrates to nitrites; utilizes citrates and causes formation of bud-pro- liferating galls on blueberry plants.