Page:Bergey's manual of determinative bacteriology.djvu/742

 Habitat: Probably sparingly distributed in soils. 5. Nocardia erythropolis (Gray and Thornton, 1928) Waksman and Henrici, 1948. {Mycobacterium erythropolis Gray and Thornton, Cent. f. Bakt., II Abt., 73, 1928, 87; Proactinomyces erythropolis Jensen, Proc. Linn. Soc. New So. Wales, 57, 1932, 371; Waksman and Henrici, in Manual, 6th ed., 1948, 898.) e.ry.thro'po.lis. Gr. adj. erythrus red; Gr.noun polis a city; M.L.noun erythropolis red city. Original description supplemented by ma- terial taken from Bynoe (Thesis, McGill University, Montreal, 1931). Long, uneven-sided rods and filaments, curved and branching, 0.8 by up to 11.0 microns. Coccoid forms not formed. Stains readily. Not acid-fast. Gram-positive. Gelatin colonies: After 12 days, round, flat, white, shining; edge entire. Deep colonies: Round, smooth. Gelatin stab: After 8 to 14 daj's, growth convex, white, smooth, shining; radiate from center; borders cleft. Line of puncture filiform, erose. Agar colonies: Round, 2 to 3 mm in diam- eter, convex, watery white; edge entire. Deep colonies: Lens-shaped. Agar slant: Filiform, flat, watery growth; edge undulate. Broth: Growth slight; turbid. Dorset's egg medium: After 2 weeks, growth raised, moist, finely granular, flesh- colored; irregular margin. LoefBer's medium: After 7 days growth as on Dorset's egg medium, but pink. Glycerol potato: After 7 days, flat, dry, rough, orange-colored. Litmus milk: Pale pink pellicle. No acid from glucose, lactose, sucrose or glycerol. Nitrites not produced from nitrates. Phenol is utilized. Optimum temperature, 25° C. Optimum pH, between 6.8 and 8.0. Distinctive characters: Differs from Nocardia coeliaca and Nocardia actino- morpha in the filiform growth and absence of liquefaction of gelatin. Long rods and filaments. Source : Six strains were isolated from soils in Great Britain. Habitat: Presumably soil. 6. Nocardia leishmanii Chalmers and Christopherson, 1916. (A new acid-fast streptothrix, Birt and Leishman, Jour. Hyg., £, 1902, 120; Chalmers and Christo- pherson, Ann. Trop. Med. and Parasit., 10, 1916, 255.) leish.ma'ni.i. M.L. gen. noun leishmanii of Leishman; named for W. B. Leishman, one of the two who first isolated this or- ganism. Description taken from Erikson (Med. Res. Council Spec. Rept. Ser. 203, 1935, 27). Initial cells frequently swollen, large and irregular, aggregated in short chains and then branching out into regular, narrow filaments; at margin of colony on synthetic glj'cerol agar may be seen comparativelj' long, thick segments with accompanying fringe of normal hyphae; later, entire colonies asteroid in appearance, very fine and close, angular branching, with aerial hyphae situated singly; aerial mycelium generally abundant with irregularly cylin- drical conidia. Slightly acid-fast. The latter property must have been attenuated during artificial cultivation, for the or- ganism is reported as markedly acid-fast by the original isolators. Gelatin: Small, pink colonies in depths of stab. No liquefaction. Glucose agar: Rounded, elevated colo- nies with paler frosting of aerial mycelium; growth becoming piled up; aerial mycelium sparse. Glycerol agar: Small, round, pink colo- nies, tending to be umbilicated and piled up; stiff, white aerial spikes. Coon's agar: Small, round, colorless colo- nies; stiff white aerial spikes; later a pink tinge. Potato agar: Minute, colorless, round colonies; small raised patches of white aerial mycelium. Dorset's egg medium: Colorless, confluent growth studded with little wart-like projec- tions bearing stiff aerial spikes; growth be- comes pinkish with a white aerial my- celium; later, growth drab gray; medium discolored.