Page:Bergey's manual of determinative bacteriology.djvu/707

 Biol., Paris, 99, 1928, 1517; Plectridio ama- rillo, Soriano, Tomo commemorativo del XXV aniversario de la fundacion de la Fa- cultad de Agronomia y Veterinaria, Buenos Aires, 1929 (?) ; Bacillus haumani (sic) Sori- ano, Rev. Inst. Bact., Buenos Aires, 5, 1930, 743; Plectridium amarillum Stampa, Ann. Brass, et Distill., 29, 1930-31, (253, 271 and 302?); Clostridium haumanni (sic) Prdvot, Ann. Inst. Past., 61, 1938, 81; Clostridium felsineum var. haumanni (sic) Heyn, Ver- hand. d. k. Akad. v. Wetensch., Anasterdam, Tweedie Reeks, 48, 1951, 29.) hau.man'i.i. M.L. gen. noun haumanii of Hauman; named for Prof. Lucien Hauman, Microbiologist of the University of Buenos Aires. Rods, averaging 0.7 to 0.8 by 4.0 to 10.0 microns, occurring singly or in short chains. Terminal, ovoid spores, 1.6 by 3.0 microns, with spore cap. Actively motile. Granulose- negative. Gram-positive, becoming Gram- negative. Gelatin: No liquefaction. Carrot agar deep colonies : Lenticular with smooth edges. Gas production disrupts agar. Intense canary-yellow pigment diffuses in medium. Carrot juice: Uniformly turbid. Potato mash : Produces good growth with slow gas and with yellow pigment produc- tion. No head formation as with Clostridium jelsinexim Bergey et al. Acid and gas from amygdalin, salicin, mannitol, arabinose, xylose, glucose, fruc- tose, mannose, galactose, sucrose, maltose, lactose, pectin and rhamnose. Arabitol, dulcitol, sorbitol, inositol, raffinose, inulin and starch not fermented. Retting action: Positive in three days. Anaerobic. Optimum temperature, 37° C. Source: Isolated from retting liquid from Argentina. Habitat: Found in decomposing plant materials. 79. Clostridium virens (Prevot, 1946) McClung and McCoy, comh. nov. {Plec- tridium virens Prevot, Ann. Inst. Past., 72, 1946, 665.) vi'rens. L. v. vireo to be green; L. part, adj. virens becoming green. Straight rods, 0.6 to 0.8 by 3.0 to 4.0 mi- crons. Terminal, spherical spores, 1 micron in diameter. Gram-positive in young cul- tures. Gelatin: No growth. Glucose agar deep colonies: Colonies ap- pear slowly in 5 to 8 days; transparent, large, woolly colonies with green, fluorescent coloration. Sometimes colonies are smaller, opaque, irregular-edged and green-pig- mented. Pigment is soluble and diffuses completely into the agar but stops at the level of the aerobic zone. VF glucose broth: Slow growth at 26° C. in 8 to 10 days; after 15 days the broth is colored green; no gas. Milk: No growth. Indole not produced. Potato mash: Strongly colored green in 2 days; marked production of gas. Lactose and galactose are strongly fer- mented. Fructose is more weakly fermented, while glucose, maltose, sucrose, sorbitol, mannitol and starch are not fermented. Cellulose is not attacked (Prevot, Man. d. Class, et d. D^term. d. Bact. Anaerob., 2« ed., 1948, 210). Propionic and formic acids are produced. Nitrites produced from nitrates. Coagulated egg white: No growth. Liver, brain and fibrin: No growth. Anaerobic. Temperature relations: Optimum, 26° C. Weak growth at 18° and no growth above 30° C. Inhibition of pigment production in second-generation cultures at 30° C. Not pathogenic for experimental animals. Source: Isolated from pond and river muds. Habitat: Presumably mud. 80. Clostridium spumarum (Prevot and Pochon, 1939) Spray, 1948. {Plectridium spumarum Prevot and Pochon, Compt. rend. Soc. Biol., Paris, 130, 1939, 966; Spray, in Manual, 6th ed., 1948, 808.) spu.ma'rum. L. noun spumn foam, froth; L. gen.pl. noun spuviarum of foams. Rods, 0.5 by 4.0 microns. Spores are ovoid and terminal, swelling the cells. Motile. Gram-positive. Gelatin: Liquefaction in 15 days.