Page:Bergey's manual of determinative bacteriology.djvu/697

 Coagulated albumin: No liquefaction. Blood serum: No liquefaction. Brain medium: No blackening or diges- tion. Beef-heart mash medium: Reddened; no blackening or digestion. Anaerobic. Optimum temperature not determined. Grows at 37° C. Not pathogenic for mice, guinea pigs or rabbits. Source: Isolated from an old culture of Clostridium parabotulinujn Type A. Habitat: Not determined. 60. Clostridium tetanoides (Adamson, 1918) Hauduroy et al., 1937. (Unnamed ana- erobe, Adamson and Cutler, Lancet, /, 1917, 688; Bacillus tetanoides (A) Adamson, Jour. Path, and Bact., £2, 1918-19, 382; Hauduroy et al., Diet. d. Bact. Path., 1937, 140.) te.ta.no.i'des. M.L. noun tetani a specific epithet; Gr. noun eidus shape, form; M.L. adj. tetanoides {Clostridium) tetani-iiQ. Rods, 1.0 to 2.0 by 4.0 to 12.0 microns (averaging 1.0 to 1.5 by 6.0 to 7.0 microns), with rounded to slightly tapered ends, occurring singly, in pairs and in chains of 3 to 5 cells but not in filaments. Spores large, spherical, terminal, swelling the cells. Motile only in young cultures. Gram- positive in young cultures, soon becoming Gram-negative. Gelatin: No liquefaction. Plain agar surface colonies (anaerobic) : Confluent, becoming an opaque film. Iso- lated colonies are circular to slightly irregu- lar. Dendritic branching and mucoid tendency less evident than on glucose agar. Glucose agar surface colonies (anaerobic) : Circular, regular, opaque, bluish gray, moist, shining, thick, raised. Surface flat, becoming conical in center with age. On moist medium, shows radiating dendritic branching. Growth becomes tenacious- mucoid. Glucose agar stab: Thick growth along stab, starting 0.5 cm below surface. No gas or splitting of medium. Neutral-red glucose agar: Reduced to orange by transmitted, and to greenish fluorescent by reflected light. Plain broth: Early, slight turbidity; clearing and mucoid sedimentation. Glucose broth: Abundantly turbid; pro- fuse, mucoid sediment. Milk: Slight and slowly increasing alka- linity; slow separation of casein. No further change. Indole produced in trace amounts in broth. Acid but no gas from glucose and maltose. Lactose, sucrose, mannitol, starch and cellulose not fermented. Coagulated albumin: No digestion or blackening. Blood serum: No digestion or blackening. Meat medium: No digestion or blacken- ing. Brain medium: No digestion or blacken- ing. Anaerobic. Optimum temperature not recorded. Grows well at 37° C. Not pathogenic for guinea pigs or rabbits. Source: Isolated from war wounds, from postmortem blood cultures and from garden soil. Habitat: Not determined. 61. Clostridium tartarivorum Mercer and Vaughn, 1951. (Jour. Bact., 62, 1951, 36.) tar.ta.ri'vo.rum. M.L. noun acidum tartaricum tartaric acid; L. v. voro to de- vour; M.L. adj. tartarivorus tartrate- destroying. Long, uniformly slender, granulated rods; older cells are slightly curved. Cells, usu- ally measuring 0.4 to 0.6 by 5.0 to 6.8 mi- crons, vary in size depending on the growth medium. Occur singly or in pairs. Spores large (0.7 to 2.0 microns), ellipsoidal to spherical and generally terminal. Sluggishly motile by means of peritrichous flagella. Gram-negative. Gelatin: No liquefaction. Green pea agar surface colonies: Large (10.5 to 12.0 mm in diameter), grayish cream in color. Colonies on tartrate agar are smaller. Indole not produced. Hydrogen sulfide not produced from tryptone (peptone); production variable from sodium thiosulfate.