Page:Bergey's manual of determinative bacteriology.djvu/686

 Not pathogenic for guinea pigs. Source: Isolated from oysters. Habitat : Found in oysters so far as known. 37. Clostridium tale (Prevot at al., 1947) McClung and McCoy, comb. nov. (In- flabilis talis Prevot, Digeon, Peyr6, Pan- taloon and Senez, Ann. Inst. Past., 73, 1947, 416.) ta'le. L. adj. talis such, so great, so ex- cellent. Straight rods, 0.7 to 0.8 by 3.0 to 5.0 mi- crons. Spores subterminal, swelling the cells. Not encapsulated. Non-motile. Gram-posi- tive. Gelatin: Liquefaction. Agar deep colonies: Lenticular with ten- dency to become irregular, evolving putrid gas. Peptone broth: Slightly turbid. VF glucose broth: Abundantly turbid; much gas; marked putrid odor. Milk: Coagulated in one to several days, then digested. Indole produced in trace amounts. Glucose, fructose, galactose, sucrose, sorbitol and glycerol fermented with the production of gas. Nitrites produced from nitrates (in pres- ence of maltose) by one strain. Coagulated protein: Slowly and partially attacked. Anaerobic. Pathogenicity variable. One strain is pathogenic for guinea pigs without pro- ducing local lesions but with hepatic degen- eration and pulmonary congestion. Toxin, on inti'avenous injection, kills mouse in several seconds. Agglutination: Sera are strain-specific. Source: Isolated from an acute appendix and from canned fish. Habitat: From decomposing organic mat- ter, so far as known. 38. Clostridium mangenotii (PrOvot and Zimmes-Chaverou, 1947) McClung and McCoy, comb. nov. {Inflabilis mangenoti (sic) Prevot and Zimmes-Chaverou, Ann. Inst. Past., 73, 1947, 603.) man . ge . no'ti . i. M.L. gen. noun mangenotii of Mangenot; named for Prof. Mangenot, director of the Institut intercolonial d'Adio- podoum6. In liquid media, short, very thick rods, 1.6 to 1.8 by 3.0 to 4.0 microns, often ovoid, with rounded ends, occurring most often in chains. On agar the rods are longer, 6.0 to 8.0 microns, often occurring in chains or filaments. Clostridial spores are formed. Non-motile. Gram-positive. Gelatin: Liquefaction in 24 hours. Agar deep colonies : Irregular, with woolly edges, sometimes arborescent; little gas. Glucose broth: Abundant, flocculating growth depositing a viscous mass. Foul odor. Milk: Digested in 24 hours. Indole and a little skatole are produced. Hydrogen sulfide abundantly produced. Glucose and maltose are fermented. Lac- tose, galactose, arabinose and starch are not attacked. Nitrites not produced from nitrates. Coagulated serum, fibrin and coagulated egg white are slowly attacked and become transparent but are not liquefied. Anaerobic. Optimum temperature, 37° C. Not pathogenic for guinea pigs. Source: Isolated from African soil. Habitat: Soil. 39. Clostridium lituseburense (Pre- vot, 1948) McClung and McCoy, comb. nov. {Inflabilis litus-eburense (sic) Prevot, Ann. Inst. Past., 74, 1948, 167.) li.tus.e.bu.ren'se. L. noun litus coast; L. noun ebur ivory; M.L. adj. lituseburensis pertaining to the Ivory Coast. Straight rods, 1.0 by 4.0 to 6.0 microns, with rounded ends, occurring in short chains. Subterminal, clostridial spores. Non-motile. Gram-positive. Gelatin: Complete liquefaction in 24 hours. Agar deep colonies: Woolly; gas is pro- duced. Peptone broth: Slightly turbid; gas. VF glucose broth: Abundantly turbid; gas; foul odor; non-coherent, slimy sedi- ment. Milk: Coagulated with rapid digestion. Indole and skatole not produced. Hydrogen sulfide produced.