Page:Bergey's manual of determinative bacteriology.djvu/654

 starch (Foster et al., Jour. Bact., 59, 1950, 463). Temperature relations: Optimum, about 37° C. Ma.ximum, about 45° C. Pathogenicity: Cause of American foul- brood of honey bees. Source: Isolated from scales of dead lar- vae. Habitat: Diseased broods. 21. Bacillus popilliae Dutky, 1940. (Jour. Agr. Research, 61, 1940, 59.) po.pil'li.ae. M.L. noun Popillia generic name of the Japanese beetle; M.L. gen. noun popilliae of Popillia. Description taken from Dutky (loc. cit.). Rods, unstained, 0.9 by 5.2 microns. Stained by crystal violet after fixing in Schaudinn's solution, 0.3 by 3.5 microns. Non-motile. Gram-positive. Spores, 0.9 by 1.8 microns, cylindrical, central. No free spores observed. Spores formed on artificial media (Steinkraus and Tashiro, Science, 121, 1955, 873). Sporangia swollen and spindle-shaped (contain a refractile body, about half the size of the spore, at the broader pole of the cell; this body reacts similarly to spores with respect to stains). Unheated egg yolk beef infusion agar slants: Small, discrete colonies. Aerobic, facultatively anaerobic. Temperature relations: Optimum, about 30° C. Maximum, about 36° C. Pathogenicity: Cause of type A milky disease of Japanese beetle (Popillia japon- ica Newm.). Source: Isolated from infected larvae. Habitat: Diseased larvae in soil. 22. Bacillus lentimorbus Dutky, 1940. (Jour. Agr. Research, 61, 1940, 65.) len.ti.mor'bus. L. adj. lentus slow; L. noun morbus disease; M.L. noun lentimorbus the slow disease. Description taken from Dutky (loc. cit.). Rods, unstained, 1.0 by 5.0 microns. Stained by crystal violet after fixing in Schaudinn's solution, 0.5 by 4.0 microns. Spores formed on artificial media (Stein- kraus and Tashiro, Science, 121, 1955, 873). Grows on artificial media (Dutky, Jour. Bact., 54, 1947, 267; also see Steinkraus and Tashiro, op. cit., 1955, 873). Aerobic, facultatively anaerobic. Temperature relations: Optimum, about 25° C. Maximum, about 30° C. Pathogenicity: Cause of type B milky disease of Japanese beetle {Popillia japon- ica Newm.). Source: Isolated from infected beetles. Habitat: Diseased larvae in soil. 23. Bacillus pantothenticus Proom and Knight, 1950. (Jour. Gen. Microbiol., 4, 1950, 539.) pan.to.then'ti.cus. M.L. adj. acidumpan- tothenicum pantothenic acid; M.L. adj. pantothenticus (probably intended to mean) related to pantothenic acid. Rods, 0.4 to 0.7 by 1.2 to 3.5 microns, not in chains. Stain evenly. Motile. Gram-posi- tive. Spores, 0.8 to 1.0 by 0.8 to 1.3 microns, round to ellipsoidal, terminal. Spore wall thin and not easily stained. (Round spores are smaller than the ellipsoidal.) Sporangia definitely swollen; drumstick- to racket-shaped. Gelatin agar streak plate: Wide zone of hydrolysis. Agar colonies: Small, round, translucent to dense, granular to moire, smooth to rough. Embedded colonies lenticular or irregular and hairy. Agar slants: Growth moderate, thick, not spreading, whitish. Glucose agar slants: Growth scant, less than on agar. Proteose-peptone acid agar slants: No growth. Soybean agar slants: No growth. Broth: Light to moderate uniform tur- bidity. NaCl broth: Growth in 10 per cent NaCl; marked stimulation by a 5 per cent concen- tration. Milk agar streak plate: Wide zone of hy- drolysis of the casein. Potato: No growth. Indole not produced. Acid but no gas (with ammonium salts as source of nitrogen) from glucose and su- crose. Acid production variable from arabi-