Page:Bergey's manual of determinative bacteriology.djvu/625

 Bergey et al., Manual, 1st ed., 1923, 158.) cel.la'se.a. M.L. adj. cellaseus pertaining to cellulose. Angular rods, 0.4 to 0.5 by 0.8 to 2.0 mi- crons, occasionall}^ clubbed, curved or branched. Motile by means of one or a few l)eritrichous Hagella. Gram-variable, ap- pearing most commonly as Gram-negative. Gelatin agar: Gelatin hydrolyzed. Gelatin stab: Ver}^ scant crateriform liquefaction in 10 days. Agar slant: Moderate, smooth, glistening, white or ivory-colored growth; lacks deep yellow pigmentation. Broth: Uniformly turbid. Filter paper in 0.5 per cent peptone broth: Paper strip reduced to a pulpy mass or weakened sufficiently so that the fibers sep- arate on slight agitation. Potato: Smooth, opaque, cream-colored growth; freshly isolated strains may fail to grow on potato. Acid from glucose, maltose, lactose, su- crose and starch; usually no acid from glyc- erol or mannitol. Acetylmethylcarbinol not produced. Starch is hydrolyzed. Nitrites produced from nitrates. Ammonia not produced. Optimum temperature, between 28° and 33° C. Source: Isolated from soil from Utah. Habitat: Probably widely distributed in soil. 3. Cellulomonas aurogena (Kellerman et al., 1913) Bergey et al., 1923. (Bacillus aurogenus Kellerman, McBeth, Scales and Smith, Cent. f. Bakt., II Abt., 39, 1913, 505; Cellulomonas aurogenes (sic) Bergey et al.. Manual, 1st ed., 1923, 157.) au.ro'ge.na. L. noun aurum gold; L. v. gigno to produce; M.L. adj. aurogenus gold (color) -producing. Cells 0.5 by 1.4 microns. Motile by means of one to three peritrichous flagella. Gram- negative. Gelatin stab: Slow liquefaction, usually not apparent until 7 to 10 days. Agar slant: Scant to abundant, flat, yel- low growth. Cellulose agar: Variable enzymatic zones surround colonies. Broth: Turbid. Potato: Scant to abundant, yellow growth. Acid from glucose, maltose, lactose, su- crose, starch and gl3'cerol. Nitrites produced from nitrates. Ammonia produced. Aerobic, facultatively anaerobic. Optimum temperature, between 28° and 33° C. Comment: A non-chromogenic variety of this species has been described by Keller- man et al. (Cent. f. Bakt., II Abt., 39, 1913, 506). Source : Isolated from soil from Louisiana and Maine. Habitat: Soil. 4. Cellulomonas pusilla (Kellerman et al., 1913) Bergey et al., 1923. (Bacillus pusilus (sic) Kellerman, McBeth, Scales and Smith, Cent. f. Bakt., II Abt., 39, 1913, 512; Cellulotiionas pusila (sic) Bergey et al., Manual, 1st ed., 1923, 161.) pu.sil'la. L. dim. adj. pusillus very small. Cells 0.4 to 0.6 by 0.8 to 1.5 microns. Mo- tile by means of one to three peritrichous flagella. Gram-negative. Gelatin stab: Slow liquefaction, usually becoming apparent after 15 days. Agar slant: Scant, grayish white growth. Cellulose agar: Variable enzymatic zones around colonies. Broth: Turbid. Potato: Scant growth when heavily inocu- lated. Acid from glucose, maltose, lactose, su- crose, starch and glycerol; no acid from mannitol. Nitrites produced from nitrates. Ammonia produced. Aerobic, facultatively anaerobic. Optimum temperature, between 28° and 33° C. Source: Isolated from soil from the Dis- trict of Columbia and from South Carolina. Habitat: Soil. 5. Cellulomonas fimi (McBeth and Scales, 1913) Bergey et al., 1923. (Bacterium fimi McBeth and Scales, U. S. Dept. Agr. Plant Ind. Bull. 266, 1913, 30; Cellulomonas fima (sic) Bergey et al.. Manual, 1st ed.,