Page:Bergey's manual of determinative bacteriology.djvu/624

 Key to the species of gemis Cellulomonas. I. Motile by means of one or a few peritrichous fiagella. A. Nitrites produced from nitrates. 1. Xylose and arabinose not fermented. a. Ammonia not produced. b. Yellow chromogenesis on nutrient agar. 1. Cellulomonas hiazotea. bb. White, grayish or ivory growth on agar. 2. Cellulomonas cellasea. aa. Ammonia produced. b. Yellow chromogenesis on nutrient agar. 3. Cellulomonas aurogena. bb. Grayish white or ivory growth on agar. 4. Cellulomonas pusilla. 2. Xylose and arabinose fermented. 5. Cellulomonas fimi. B. Nitrites not produced from nitrates. 1. Yellow chromogenesis on nutrient agar. 6. Cellulomonas galba. 2. Grayish white or ivory growth on agar. 7. Cellulomonas gelida. II. Non-motile. A. Nitrites produced from nitrates. 1. Yellow chromogenesis on nutrient agar. 8. Cellulomonas flavigena. 2. Grayish white or ivory growth on agar. 9. Cellulomonas uda. B. Nitrites not produced from nitrates. 10. Cellulomonas acidula. 1. Cellulomonas biazotea (Kellerman Paper strip reduced to a pulpy mass or et al., 1913) Bergey et al., 1923. {Bacillus weakened sufficiently so that the fibers hiazoteus Kellerman, McBeth, Scales and separate on slight agitation. Smith, Cent. f. Bakt., II Abt., 39, 1913, 506; Potato: Smooth, yellow growth. Bergey et al.. Manual, 1st ed., 1923, 158.) Acid from glucose, lactose, maltose, su- bi.az.o'te.a. L. prefix hi-iwo; Gr. azous crose and glj'cerol; no acid from mannitol. without life; Fr. noun azote nitrogen; M.L. Acetylmethylcarbinol not produced, adj. hiazoteus denoting two nitrogen sources Starch is hydrolyzed. utilized {i.e., organic and inorganic). Nitrites produced from nitrates. Small, angular rods 0.5 by 0.8 to 1.5 mi- Ammonia not produced, crons, occasionally branched, beaded. Aerobic, facultatively anaerobic, clubbed or curved. Motile by means of one Optimum temperature, between 28° and or a few peritrichous flagella. Gram-varia- 33° C. ble, appearing most commonly as Gram- Source: Isolated from soil from Utah, negative. Habitat: Soil. Probably widely distribu- Gelatin agar: Gelatin hydrolyzed. ted in soil and in decomposing plant resi- Gelatin stab: Slow, crateriform liquefac- dues, tion in 10 days. Agar slant: Moderate, smooth, opaque, 2. Cellulomonas cellasea (Kellerman yellow growth. et al., 1913) Bergey et al., 1923. {Bacillus Broth: Uniformly turbid. cellaseus Kellerman, McBeth, Scales and Filter paper in 0.5 per cent peptone broth: Smith, Cent. f. Bakt., II Abt., 39, 1913, 508;