Page:Bergey's manual of determinative bacteriology.djvu/618

 137, 1953, 1-10) found a culture of this organ- ism isolated from a submandibular abscess to be identical with C. liquefaciens as recog- nized by Prevot and also identical with cultures of C. acnes as isolated and identi- fied by Lentze (Zent. f. Bakt., I Abt., Orig., 155, 1950, 290). Since that time, Seeliger (personal communication, December, 1954) has had opportunity to study the cultures of C. acnes found in both the National Col- lection of Type Cultures (London) and the American Type Culture Collection (Wash- ington), all isolated from acne pustules, and he has found all of them to be identical. At the same time he finds these cultures to be the same as the cultures that he examined in 1953. All cultures agree with the brief but characteristic description of C. acnes as given by Gilchrist. As the name C. acnes has priority and as the name C. liquefaciens is an illegitimate homonym, the former binomial is used here. Source: Originally isolated from acne pustules. The specific epithet "acnes" was given by Gilchrist to indicate the source of this culture, and it should not be interpreted as meaning that this species is the cause of acne. Habitat : While this species appears to be an organism commonly found in acne pus- tules, it also occurs in other types of lesions in the human body or even as a saprophyte in the intestine, in skin, in hair follicles and in sewage. It probably also occurs in domes- tic and wild animals. 30. Corynebacteriuni parvuni Prevot, 1940. {Corynebacterium parvum injectiosum Mayer, Cent. f. Bakt., I Abt., Orig., 98, 1926, 370; Prevot, Man. de Class, et Determ. des Bacteries Anaerobies. Monographic, Inst. Past., Paris, 1940, 202.) par'vum. L. adj. parvus small. Small, slightly curved, club-shaped rods, usually 0.3 by 1.4 microns, occurring in pairs in which the cells are either parallel to each other or in an angular arrangement due to snapping division. Non-motile. Gram- positive. Gelatin: No liquefaction. Nutrient broth: Slightly turbid. Carbohydrates generally not attacked; slight acid from glucose. Neutral red not reduced. Blood serum agar: Numerous, porcelain- w^hite colonies. Obligately anaerobic. Pathogenic to white mice. Serology: See Linzenmeier (Ann. Inst. Past., 87, 1954, 572). Source : Isolated from blood from a woman with a postnatal fever. Also found in various infections of the female urogenital organs. Habitat: Found in female urogenital or- gans so far as known. 31. Corynebacterium granulosum Pre- vot, 1938. (Bacille granuleux, Jungano, Compt. rend. Soc. Biol., Paris, 66, 1909, 123; Prevot, Ann. Inst. Past., 60, 1938, 304.) gra.nu.lo'sum. L. noun granula a little grain; L. adj. granulosus full of granules. Slender, medium-sized rods resembling those of Corynebacterium diphtheriae. Non- motile. Show bipolar staining and meta- chromatic granules. Gram-positive. Gas not produced in culture media. Gelatin: No liquefaction. Agar stab: Round colonies; no gas. Glucose broth: Turbid; no gas. Milk: Not coagulated. Coagulated egg white: Not attacked. Glucose and galactose are acidified. Anaerobic. Optimum temperature, 37° C. Not pathogenic. Serology: See Linzenmeier (Ann. Inst. Past., 87, 1954,572). Source: Isolated from the intestines of white rats. 32. Corynebacteriuni aviduni (Eggerth, 1935) Prevot, 1938. (Bacteroides avidus Eg- gerth, Jour. Bact., SO, 1935, 289; Prevot, Ann. Inst. Past., 60, 1938, 304.) a'vi.dum. L. adj. avidus greedy, vora- cious. Rods, 0.5 to 1.0 by 1.0 to 2.5 microns, with pointed or rounded ends, frequently slightly curved, occasionally branched. Non-motile. Gram-positive. Gelatin liquefied. Glucose agar colonies: 2 to 3 mm in di- ameter, raised, yellowish white, smooth. Glucose broth: Turbid; acidified (final pH, 4.8).