Page:Bergey's manual of determinative bacteriology.djvu/609

 ferment fructose and mannose (Merchant, Jour. Bact., 30, 1935, 109). Aerobic, facultatively anaerobic. Optimum temperature, 37° C. Pathogenic for mice (Lovell and Cotchin, Jour. Comp. Path, and Therap., 56, 1946, 205) and for rabbits (Feenstra, Thorp and Gray, Amer. Jour. Vet. Res., 10, 1949, 12). No to.xin produced. Shows a close serological relationship with Corynebacteriurn psuedotuberculosis (Mer- chant). Source: Isolated from pyelonephritis in cattle. Habitat: Occurs in purulent infections of the urinary tract in cattle, sheep, horses and dogs. 10. Corynebacteriurn kutscheri (Mi- gula, 1900) Bergey et al., 1925. {Bacillus pseudotuberculosis murium Kutscher, Ztschr. f. Hyg., 18, 1894, 338; Bacterium kutscheri Migula, Syst. d. Bakt., 2, 1900, 372; Bergey et al.. Manual, 2nd ed., 1925, 395.) kut'scher.i. M.L. gen. noun kutscheri of Kutscher; named for Kutscher, the bac- teriologist who first isolated this species. Rods, with pointed ends, staining irregu- larly. Non-motile. Gram-positive. Gelatin colonies: Small, white, translu- cent. Gelatin stab: No growth on surface. White, filiform growth in stab. No lique- faction. Agar colonies: Small, thin, yellowish white, translucent, serrate. Agar slant: Thin, white, translucent. Loeffler's blood serum: Abundant growth. Not peptonized. Broth: Slight turbidity. Crystals of am- monium magnesium phosphate are pro- duced. Litmus milk: Unchanged. Potato: No growth. Indole not produced. Nitrites not produced from nitrates. Aerobic, facultative. Optimum temperature, 37° C. Source: Isolated from a cheesy mass in the lung of a mouse. 11. Corynebacteriurn phocac Sven- kerud et al., 1951. (Svenkerud, Rosted and Thorshang, Nord. Vet. Med., S, 1951, 168.) pho'cae. M.L. noun Phoca a generic name of seals; M.L. gen. noun phocae of Phoca. Description prepared by Prof. E. G. D. Murray from the original publication by Svenkerud et al. and from a study of cul- tures supplied by these investigators. Rods, 0.4 to 0.6 by 0.7 to 2.0 microns, oc- curring in frequently flexed chains of 3 to 7 or more cells, in linear, end to end pairs or in pairs lying at an obtuse angle to each other; occasionally single cells and very long rod-shaped forms, 10 to 15 microns in length, may occur, the latter sometimes being curved. Non-motile. Gram-positive. Gelatin: No liquefaction. Agar colonies: Small (0.1 mm), circular, smooth, moderately elevated, entire; at first (24 hours) transparent and colorless, later (48 hours) becoming enlarged (0.5 to 1.0 mm), opalescent and slightly white. Potassium tellurite medium: No growth. Peptone broth: Poor growth at 37° C. and room temperature. Pneumo broth: Good growth at 37° C. and room temperature; slight growth in 3 to 4 days at 4° C., becoming progressively heavier and producing a turbidity in the lower portion of the medium, thus leaving a clear supernatant several mm in depth. Coagulated blood serum: No liquefaction. Litmus milk: No change. Indole not produced. Hydrogen sulfide not produced. Carbohydrate fermentation determina- tions (except those of esculin, arbutin and alpha-methyl-glucoside) performed in Hiss serum sugar water. Acid from glucose, fruc- tose, sucrose, maltose, trehalose and salicin. Slight acid from xj-lose, galactose, lactose, inulin, glycerol, inositol and mannitol. Acid occasionally produced from rhamnose and dextrin. No acid from arabinose, dulcitol, sorbitol or arbutin. Glucose, fructose, su- crose and salicin cause clotting of the me- dium in 24 hours at 37° C. as does trehalose in 48 hours; dextrin causes clotting l)y one week. Starch, aesculin and alpha-met h3-l-glu- coside are hydroljzed. Methyl red test positive. Acetylmethylcarbinol produced.