Page:Bergey's manual of determinative bacteriology.djvu/608

 transmitted light and bluish white by re- flected light. Bovine blood serum slants: Pit-like or more general areas of liquefaction. Serum bouillon: Cloudy with fine floccu- lent grayish flakes that form a sediment like a streptococcus culture. Milk: Coagulation after 48 hours at 37° C, with acid at bottom of tube. Separation of whey and peptonization. Indole not produced. Acid produced in serum bouillon from glucose, sucrose, lactose and xylose but not from raffinose, inulin, mannitol or sali- cin. Nitrites not produced from nitrates (Mer- chant, Jour. Bact., 30, 1935, 108). Beta hemolytic; not hemoglobinophilic, though growth is favored by proteins as egg albumen, serum or blood (Brown and Orcutt, op. cit., 1920, 244). Aerobic as well as anaerobic growth. Optimum temperature, 37° C. Growth range, 20° to 40° C. Intravenous injection of rabbits and mice usually fatal. Toxin and a heat-labile hemolysin are produced (Lovell, Jour. Path, and Bact., 45, 1937, 339). Source: Isolated from bovine pus (Lucet, op. cit., 1893, 327); also isolated from path- ological processes in man (Forgeot, Halbron and Levy-Bruhl, Ann. Inst. Past., 65, 1940, 326; also see Ballard, Upsher and Seeley, Amer. Jour. Clin. Path., 27, 1947, 209). Habitat: Found in abscesses in cattle, swine and other warm-blooded animals, including man. 8. Corynebacterium murisepticuni von Holzhausen, 1927. (Cent. f. Bakt., I Abt., Orig., 105, 1927, 94.) mu.ri.sep'ti.cum. L. noun »ius, muris a mouse; Or. adj. septicus septic; L. adj. tnuri- septicvs mouse-poisoning (-infecting). Slender rods, 1.2 to 1.5 microns in length, with polar granules. Grow out into long filaments. Non-motile. Gram-positive. Gelatin stab: Feeble growth, with fimbri- ate outgrowth along line of puncture. Egg glycerol broth: Good growth. Loeflfler's blood serum: Good growth. Broth: Turbid. Litmus milk: Acid. No coagulation. Potato: Good growth. Indole not produced. Hydrogen sulfide produced. Acid from glucose, fructose, galactose, maltose, lactose, sucrose, inulin and man- nitol. Arabinose and isodulcitol are not attacked. Action on nitrates not reported. Aerobic, facultative. Optimum temperature, 37° C. Pathogenic for mice. Habitat: Septicemia in mice. 9. Corynebacterium renale (Migula, 1900) Ernst, 1905. (Bacillus renalis bovis Bollinger, in Enderlen, Zeit. f. Tiermed., 17, 1890, 346; Bacterium renale Migula, Syst. d. Bakt., 2, 1900, 504; Ernst, Cent. f. Bakt., I Abt., Orig., 40, 1905, 80.) re.na'le. L. adj. rewaZz's pertaining to the kidne^^s. Description taken mainlj^ from Jones and Little (Jour. Exp. Med., U, 1926, 11). Rods, 0.7 b}' 2.0 to 3.0 microns, occurring usually in masses, rarely singly. Non-motile. Bacteria from tissues not so pleomorphic as those from the earlier transfer cultures, although many show polar granules or swol- len ends. Cultures grown in broth show coc- coid forms and beaded rods with swollen ends. Gram-positive. Gelatin: Grows poorly if at all. No lique- faction. Agar: Small, punctiform colonies. Agar slants: Raised, grayish white and dry growth (Jones and Little). Others say cream-colored and moist. Blood serum slants: Fine, gray, puncti- form colonies in 24 hours at 37° C. which are a little larger than those on agar. Streak scarcely 1 mm in width. Glistening and slimy in fresh cultures. No liquefaction. Litmus milk: Reduction and coagulation from the bottom. Slow digestion, becoming alkaline. Broth: Sediment at end of 2 days with clear bouillon above. Potato: Growth grayish white, later be- coming a dingy yellow, turning the potato brown. Acid from glucose. No acid from lactose, sucrose, maltose or mannitol. Some strains