Page:Bergey's manual of determinative bacteriology.djvu/590

 Orig., 59, 1911, 101 ; Bacieroides tenuis Bergey et al., Manual, 1st ed., 1923, 263; Cillobac- terium spatulif or me Frevot, Ann. Inst. Past., 60, 1938, 297.) te'nu.e. L. adj. tenuis slender. Straight or curved rods with square ends; one of the ends is usually enlarged, thus giving the cells the appearance of spatulas. Motile. Gram-positive. Gas produced in culture media. Gelatin: Liquefaction. Deep agar colonies: Circular. Small amount of gas is produced. Milk: Coagulated then digested. Coagulated protein is attacked. Indole is produced. Acid and gas from glucose. Anaerobic. Optimum temperature, 37° C. Pathogenicity unknown. Source: Isolated from feces from a dog. Habitat: Unknown. 5. Cillobacterium multiforme (Dis- taso, 1911) Prevot, 1938. (Bacillus multi- formis Distaso, Cent. f. Bakt., I Abt., Orig., 59, 1911, 101; not Bacillus jtiultiformis van Senus, Dissert., Leiden, 1890; Prevot, Ann. Inst. Past., 60, 1938, 297.) mul.ti.for'me. L. adj. multus much, many; L. noun /orma shape; M.L. adj. 7nul- tiformis many-shaped. Straight or curved, thick, pleomorphic rods, 3 microns long. Motile. Gram-positive. Gas and odor produced in culture media. Gelatin: Liquefaction. Deep agar colonies: Lenticular. Abundant gas is produced. Glucose broth: Turbid. Gas is produced. Putrid odor. Milk: Coagulated then digested. Coagulated protein is attacked. Fibrin and coagulated serum are digested. Indole is produced in trace amounts. Acid and gas from glucose, lactose, fruc- tose, maltose, galactose, sorbitol, mannitol and glycerol. Ammonia, hydrogen sulfide and formic, butyric and lactic acids are produced. Nitrites not produced from nitrates. Neutral red reduced. Anaerobic. Optimum temperature, 37° C. Neither toxin nor hemolysin is pro- duced. Not pathogenic for guinea pigs. Source: Isolated from the feces of a dog; also from soil from Equatorial Africa. Habitat: Unknown. 6. Cillobacterium combesii Prevot and Laplanche, 1947. (Ann. Inst. Past., 73, 1947, 687.) com.be'si.i. M.L. gen. noun combesii of Combes; named after Combes. Straight rods, 0.7 by 3.0 to 4.2 microns, with square ends, occurring singly or in pairs, more often in chains of 3 to 10 ele- ments. Motile by means of slow undula- tions. Gram-positive. Gas and odor produced in culture media. Gelatin: Liquefaction in 5 days. Deep agar colonies: Irregular; arbores- cent or like tufts of cotton. Gas is produced. Glucose broth : Abundant turbidity which forms a viscous, zoogloea-like mass. Fetid gas is produced. Peptone broth: Turbid. Milk: Coagulated in 8 days, then digested. Coagulated proteins not attacked. Carbohydrates not attacked. Ammonia, hydrogen sulfide, alcohol, traces of acetylmethylcarbinol and formic, butyric and valerianic acids are produced. Nitrites not produced from nitrates. Neutral red and safranin are reduced. Anaerobic. Optimum temperature, 37° C. Toxin not produced. Not pathogenic for guinea pigs or mice. Source: Isolated from forest soil from French West Africa. Habitat: Unknown.