Page:Bergey's manual of determinative bacteriology.djvu/578

 8. Eubacteriuni quintum Prevot, 1940. (Anaerob No. V, Rodella, Ztschr. f. Hyg., U, 1902, 475; Prevot, Man. d. Classif. et d. Determ. d. Bact. Anaerobies, 1940, 65.) quin'tum. L. adj. quintus fifth. More or less thick rods, of variable length, with rounded ends. Non-motile. Gram-posi- tive. Gas but no odor produced in culture me- dia. Gelatin: Liquefaction in 5 to 7 days. Deep agar colonies: Opaque centers. Gas is produced. Glucose broth: Rapid turbidity. Milk: Digested in 2 to 3 days after coagu- lation. Coagulated serum slowly digested. Anaerobic. Pathogenic. Guinea pigs killed in 48 hours after subcutaneous injection. Source: Isolated from cases of infantile diarrhea. Habitat: Found in the intestines of chil- dren. Uncommon. 9. Eubacteriuni liiiiosum (Eggerth, 1935) Prevot, 1938. (Bacteroides limosus Eggerth, Jour. Bact., 30, 1935, 290; Prevot, Ann. Inst. Past., 60, 1938, 295.) li.mo'sum. L. adj. limosus full of slime, slimy. Rods, 0.5 to 1.5 by 1.0 to 5.0 microns; the average length is 3.0 to 4.0 microns. Pleo- morphic. Occur as short ovoids and as wedge-shaped bacilli; curved, hooked and bifid forms are numerous. Metachromatic granules are absent. Non-motile. Gram- positive. Gas but no odor produced in culture me- dia. Gelatin: Slow liquefaction. Glucose agar and blood agar colonies: 2 to 4 mm in diameter, raised, cream-colored, mucoid, adherent. No hemolysis on blood agar. Glucose broth: Cloudy with a heavy, mucoid sediment; the pH reaches 4.8. Milk: Unchanged. Coagulated proteins not attacked. Indole not produced. Hydrogen sulfide not produced. Acid and gas from glucose, fructose, man- nitol, adonitol, erythritol, de.xtrin and tre- halose. A slight acidity (pH 6.0 to 6.5) de- velops without detectable gas in aesculin, cellobiose, glucosamine, inulin, glycogen, maltose, mannose, methylmannoside, raffi- nose, salicin, starch and sucrose. No acid from amygdalin, arabinose, dulcitol, galac- tose, glycerol, inositol, lactose, melezitose, methylglucoside, rhamnose, sorbitol or .xjdose. Gas and volatile acid, of which butyric acid is a main component, are produced from glucose; traces of lactic acid are also produced (Pederson, Jour. Bact., 50, 1945, 478). Nitrites not produced from nitrates. Anaerobic. Optimum temperature, 37° C. Non-pathogenic for white mice and rab- bits. Relationship to other species: A culture of this organism supplied to Dr. H. A. Bar- ker has been carefully studied. Barker (per- sonal communication, November, 1955) states that acetic and n -butyric acids are produced from glucose and lactate and that this organism should be transferred to the genus Butyribacterium. Source: One strain, obtained as a single- cell culture, was isolated from human feces. Habitat: Found in human feces and pre- sumabl}^ in the feces of other warm-blooded animals. 10. Eubacteriuni ethylicum Prevot, 1938. {Bacillus gracilis ethylicus Achalme and Rosenthal, Compt. rend. Soc. Biol., Paris, 58, 1906, 1025; Prevot, Ann. Inst. Past., 60, 1938, 295.) e.thy'li.cum. Gr. noun ether ether; M.L. neut.n. ethyl the ethyl radical; M.L. adj. ethylicus pertaining to ethyl. Slender, straight or curved rods occurring singly, in pairs or in short chains. Granular. Non-motile. Gram-positive. Gas but no odor produced in culture me- dia. Gelatin: No liquefaction. Deep agar colonies: At first punctiform, spreading to 2 mm in diameter; irregular. Glucose broth: Flocculent growth which precipitates, leaving the medium clear. Ammonia is produced. Peptone broth: Same as for glucose broth.