Page:Bergey's manual of determinative bacteriology.djvu/577

 Jtl, 1902, 474; Pr6vot, Ann. Inst. Past., 60, 1938, 294.) quar'tum. L. adj. quartus fourth. Thick rods, with rounded ends, which vary in length. Non-motile. Gram-positive. Gas and fetid odor produced in culture media. Gelatin: Liquefaction in 6 to 9 days. Sedi- ment. Deep agar colonies : Small, round, arbores- cent. Glucose broth: Turbid. Abundant gas. Sediment. Milk: Digested in 3 to 5 days. Cheese-like odor produced. Coagulated serum not liquefied. Hydrogen sulfide is produced. Acid from glucose, fructose, maltose and glycerol. Ammonia, volatile amines, alcohol and formic, butyric and lactic acids are pro- duced. Nitrites not produced from nitrates. Neutral red and safranin are reduced. Anaerobic. Optimum temperature, 37° C. Killed at 70° C. Pathogenic. Guinea pigs killed in 24 hours by intraperitoneal inoculation. Source: Isolated from feces in cases of infantile diarrhea; also from soil from French West Africa. Habitat: Found in intestines of children. Rather uncommon. 6. Eubacterium pseiitlotortuosuiii Pr6- vot, 1947. (Ann. Inst. Past., 73, 1947, 409.) pseu.do.tor.tu.o'sum. Gr. adj. pseudes false; L. adj. tortuosus full of windings, a specific epithet; M.L. adj. pseudotortuosum not the true {Eubacterium) tortuosum. Straight or curved rods, 0.4 to 0.5 by 3.0 to 4.0 microns, occurring in twisted chains or wavy filaments. Non-motile. Gram-posi- tive (decoloring easily). Gas but no odor produced in culture me- dia. Gelatin: Liquefaction in 3 days. Deep agar colonies: Lenticular. Gas is produced. Glucose broth: Abundant turbidity and gas. Peptone broth: Turbid. Milk: No coagulation. Coagulated proteins not attacked. Hydrogen sulfide is produced. Acid from glucose, fructose, maltose, sucrose, lactose, galactose, sorbitol and starch. Ammonia, formic, butyric and lactic acids, volatile amines, alcohol, aldehyde, ketone and acetylmethylcarbinol are pro- duced. Nitrites produced from nitrates. Neutral red and safranin are reduced. Anaerobic. Optimum temperature, 37° C. Optimum pH, 7.4. Not pathogenic for guinea pigs or mice. Source: Isolated from a case of purulent, acute appendicitis. Habitat: Found in human intestines. Un- common. 7. Eubacterium tortuosum (Debono, 1912) Pr^vot, 1938. (Bacillus tortuosus De- bono, Cent. f. Bakt., I Abt., Orig., 6£, 1912, 233; Pr^vot, Ann. Inst. Past., 60, 1938, 295.) tor.tu.o'sum. L. adj. tortuosus full of windings. Straight rods of medium size, with rounded ends, occurring in twisted chains. Non-motile. Gram-positive. Slight gas production in culture media. Gelatin: Granular growth. No liquefac- tion. Deep agar colonies: Small, lenticular or irregular, grayish. Little gas produced. Glucose broth: Uniform turbidity. Vis- cous, coherent sediment. Gas produced. Milk: Acidified; no coagulation. Coagulated proteins not digested. Indole not produced. Hydrogen sulfide not produced. Acid and gas from glucose, lactose, su- crose, galactose, fructose, maltose, manni- tol and starch. Ammonia, acetylmethylcarbinol and for- mic, propionic and lactic acids are produced. Nitrites not produced from nitrates. Neutral red reduced. Anaerobic. Not pathogenic. Source: Isolated from human feces. Habitat: Found in the human intestine. Rather uncommon.