Page:Bergey's manual of determinative bacteriology.djvu/521

 which is fixed in position, a station; L. gen. noun slationis of a fixed position. Ovoid rods, 0.4 by 0.5 to 0.6 micron, occurring singly or in chains of two to three. Non-motile. Gram-positive, but easily de- stained. All media except the fresh-water broth, litmus milk and potato were prepared with sea water. Gelatin colonies: 0.5 to 1.0 mm in diame- ter, circular, convex, grayish white. Gelatin stab: Very slow, napiform lique- faction. Agar colonies: 1 to 2 mm in diameter, convex, smooth, colorless; lobate edge. Agar slant: Moderate, glistening, filiform, butyrous growth with no pigment. Sea-water broth: Heavy pellicle; no tur- bidity; granular growth along walls; scant sediment. Fresh-water broth: Good growth. Litmus milk: Becomes alkaline. Casein not digested. Potato: No visible growth. Indole not produced. Hydrogen sulfide not produced. Acid but no gas from glucose. Lactose, maltose, sucrose, mannitol, glycerol, xylose and salicin not utilized. Starch not hydrolyzed. Non-lipolytic. Of 19 amino acids tested, none was re- quired for growth; preformed growth factors also were not required (Campbell and Wil- liams, Food Research, 16, 1951a, 506). Ammonium chloride and the 19 amino acids which were tested may serve as sources of nitrogen; the amino acids may also be utilized as carbon sources (Campbell and Williams, loc. cit.). Nitrites rapidly produced from nitrates. Ammonia produced from peptone but not from urea. Trimethylamine not produced from tri- methjiamine oxide, betaine, choline or acetyl choline (Campbell and Williams, Jour. Bact., 62, 1951b, 250). Inorganic sulfur may serve as a source of sulfur (Campbell and Williams, op. cit., 1951a, 506). Aerobic, facultatively anaerobic. Optimum temperature, between 20° and 25° C. Source: Isolated from a film of marine fouling organisms. Habitat: Sea water.

15. Brevibacterium quale (Steinhaus, 1941) Breed, 1953. (Bacterium qualis (sic) Steinhaus, Jour. Bact., 42, 1941, 774; Breed, Riassunti delle Comunicazioni, VI Con- gresso Internaz. di Microbiol., Roma, 1, 1953, 13; also see Atti del VI Congresso Internaz. di Microbiol., Roma, 1, 1955, 13.) qua'le. L. proun.adj. qualis of what sort? Short rods, very short on solid media, frequently ellipsoidal in shape. In fluid media, 0.5 to 0.7 by 1.4 to 2.2 microns, occurring singly. Non-motile. Gram-posi- tive. Gelatin stab: Liquefaction. Agar colonies: Small (1 mm), white, glis- tening, transparent, circular, entire. Agar slant: Filiform, smooth, glistening. Broth: Almost clear; slight turbidity in serum and glucose broth. Litmus milk: No change. Indole not produced. Hydrogen sulfide not produced. Acid from glucose, sucrose and maltose. Lactose not fermented. Starch not hydrolyzed. Slight production of nitrites from ni- trates. Source: Isolated from the alimentary tract of the tarnished plant bug, Lygus pratensis L. Habitat: Unknown.

16. Brevibacterium ammoniagenes (Cooke and Keith, 1927) Breed, 1953. (Bac- terium ammoniagenes Cooke and Keith, Jour. Bact., 13, 1927, 315; Breed, Riassunti delle Comunicazioni, VI Congresso Inter- naz. di Microbiol., Roma, 1, 1953, 14; also see Atti del VI Congresso Internaz. di Microbiol., Roma, 1, 1955, 14.) am.mo.ni.a'gen.es. M.L. noun ammonia ammonia, from Gr. noun Amnion (Jupiter), worshipped in Egypt in the form of a ram; Gr. V. gennaio to produce; M.L. adj. am- moniagenes ammonia producing. Rods with rounded ends, 0.8 by 1.4 to 1.7 microns, occurring singly. Not encapsu- lated. Non-motile. Gram-positive. Gelatin stab: No liquefaction.