Page:Bergey's manual of determinative bacteriology.djvu/514

 3. Nitrite production not recorded. 17. Brevibacierium healii. II. Motile. A. Gelatin not liquefied. 1. Grayish white on agar slant. 18. Brevibacierium incertum. 2. Pinkish orange to j'ellow on agar slant. 19. Brevibacierium imferiale. B. Gelatin liquefied. 1. Grayish white on agar slant. 20. Brevibacierium lipolylicum. 2. Yellow to yellow-brown on agar slant. a. Orange chromogenesis on potato. 21. Brevibacierium acelylicum. aa. Yellow chromogenesis on potato. b. Sulfur-yellow streak on potato. 22. Brevibacierium sulftireum. bb. Abundant, thickened, yellow growth on potato. 23. Brevibacierium helvolum. 1. Brevibacierium linens (Weigmann, 1910) Breed, 1953. (Organismus IX, Wolff, Milchwirt. Zent., 5, 1909, 145; Baclerium linens Weigmann, in Wolff, Cent. f. Bakt., II Abt., 28, 1910, 422, and in Weigmann, Mykologie der Milch, 62, 1911, 220; Breed, Riassunti delle Comunicazioni, VI Con- gresso Internaz. di Microbiol., Roma, 1, 1953, 13; also see Atti del VI Congresso Internaz. di Microbiol., Roma, 1, 1955, 13.) li'nens. L. part. adj. linens spreading over, smearing. Also see Steinfatt (Milchwirt. Forsch., 9, 1930, 7), Kelly (Jour. Dairy Sci., 20, 1937, 239) and Albert, Long and Hammer (Iowa Agr. Exp. Sta. Res. Bui. 328, 1944). Rods. Average 0.6 by 2.5 microns when grown 1 to 2 days on tryptone glucose ex- tract agar. Non-motile (Wolff, op. cit., 1909, 145). Gram-positive (Kelly, op. cit., 1937, 239). Gelatin colonies: At 18° C., punctiform at first; after 12 daj^s about 1 mm in diameter, compact, circular, shiny, brownish yellow to red-brown. Liquefaction. Gelatin stab: At 21° C., crateriform li- quefaction, becoming infundibuliform on extended incubation. Rate of liquefaction varies considerably with different cultures, some completing it in 15 days, others not completing it even on long incubation. Agar colonies: On tryptone glucose ex- tract agar at 21° C. after 1 to 2 days, colonies convex, glistening, entire and cream- colored, becoming brown on extended in- cubation; diameters 2 to 5 mm. On special cheese agar with incubation in oxygen, luxuriant growth, the color becoming bright orange to reddish brown in 4 or 5 days. Agar stab: Heavy surface growth on tryptone glucose extract agar at 21° C. with no growth along the line of inoculation. Agar slant: On tryptone glucose extract agar at 21° C. after 2 days, growth abun- dant, glistening, filiform, non-viscid and cream-colored. After extended incubation the color usually is brown. On special cheese agar in an atmosphere of oxygen, the growth is bright orange to reddish brown in 4 or 5 days. Broth: Turbidity and sediment. Potato: At 21° C. after 5 days, growth is scant, smooth, glistening and varies in color from grayish to brownish orange. Litmus milk: At 21° C. the changes are very slow. After 6 or 7 days the reaction be- comes alkaline and a yellow sediment ap- pears. After approximately 10 days some digestion is evident, complete digestion gen- erally requiring several weeks to over a month. A distinct ammoniacal odor, more or less objectionable, produced in old cul- tures. No coagulation. Ropiness often pro- duced on extended incubation.