Page:Bergey's manual of determinative bacteriology.djvu/500

 (Ann. Sci. Nat., Ser. Bot. et Zool., 15, 1933, 158). Spheres, 0.5 to 0.6 micron in diameter, occurring in pairs and tetrads, rarely in very short chains, never in clu.sters. Gram-posi- tive. Gehitin: Good growth. No liquefaction. Deep agar colonies: At first very small, lenticular, biconvex, thick, opaque, yel- lowish, 0.5 to 1.5 mm in diameter. Each colony is surrounded by many small satel- lite colonies visible microscopically. Broth: Growth slow, poor. After 48 hours a slight, homogeneous turbidity is formed; it quickly clears leaving a slight, powdery sediment. Neither gas nor odor is produced. Glucose broth: Growth rapid, abundant. Proteins not attacked. Blood broth: Good growth; no hemolysis. Milk: Poor growth; no change. Peptone water: Good growth; not acidi- fied; indole not produced. Acid but no gas from glucose and arabi- nose. Slight acid from glycerol. No acid from lactose, inulin, mannitol or dulcitol. Neutral red broth: Unchanged. Anaerobic. Optimum temperature, 37° C. Feeble growth at 22° C. Not thermo-resistant. Optimum pH, between 6.0 and 8.0. Distinctive character: The microscopic appearance of agar colonies, each of which is surrounded by a constellation of satel- lites. Source: Isolated from a case of chronic, cryptic tonsillitis; later isolated from pus in acute appendicitis. Habitat: Found in the digestive tract, es- pecially the lymphoid tissues, such as ton- sils, etc. 8. Peptococcus saccharolyticus (Fou- bert and Douglas, 1948) Douglas, comb. nov. {Micrococcus saccharolyticus Poubert and Douglas, Jour. Bact., 56, 1948, 30 and 31.) sac.cha.ro.ly'ti.cus. Gr. noun sacchar sugar; Gr. adj. lyticus able to loose; M.L. adj . saccharolyticus sugar-digesting. Spheres, 0.6 to 1.0 micron in diameter, occurring singly, in pairs, tetrads and ir- regular groups. Non-motile. Not encapsu- lated. Gram-positive. Gelatin: No liquefaction. Agar colonies: Circular, 0.5 to 1.0 mm in diameter, smooth, entire, low convex, opaque, grayish white, butyrous. Growth in fluid medium: In peptone yeast extract broth, growth is moderate and stimulated by glucose. Cultures are turbid with a white, powdery sediment. No visible gas production. Faint, fruity odor. Litmus milk: Slight reduction of litmus. Indole not produced. Hydrogen sulfide not produced. Acid but no visible gas from glucose, fructose, mannose and glycerol. Arabinose, galactose, maltose, lactose, raffinose, starch, inulin, salicin, mannitol, lactate and malate not utilized. Glucose fermented to CO2 , ethanol, acetic acid, formic acid and traces of lactic acid. Nitrites produced from nitrates; nitrites are reduced. Egg albumen, beef serum and casein not attacked. Coagulase-negative. Catalase-positive. Anaerobic. Optimum temperature, 37° C. Growth between 25° and 37° C. Optimum pH, between 7.0 and 7.5; pH range, 5.5 to 8.5. Non-hemolytic. Distinctive characters: Saccharolytic; no visible gas produced; characteristic fer- mentation products from glucose. Comments: There seems to be little dif- ference between this and the preceding species. Source: Isolated from bottles of plasma; also from the human skin. Habitat: Probably the skin of man. 9. Peptococcus glycinophilus (Cardon and Barker, 1946) Douglas, comb. nov. {Diplococcus glycinophilus Cardon and Barker, Jour. Bact., 52, 1946, 629.) gly.ci.no'phi.lus. M.L. noun glycinum glycine; Gr. adj. philus loving; M.L. adj. glycinophilus glycine-loving. Original description supplemented by material from Foubert (Thesis, University of Washington, 1947). Spheres, 0.7 to 2.5 microns in diameter, the average being about 1.2 microns, occa- sionally rod-shaped, occurring in pairs,