Page:Bergey's manual of determinative bacteriology.djvu/479

 mann and Neumann, Bakt. Diag., 1 Aufi., S, 1896, 161.) lu'te.us. L. adj. luteus golden-yellow. Spheres, 1.0 to 1.2 microns in diameter, occurring in pairs and fours. Non-motile. Gram-positive. Gelatin colonies: Yellowish white to yel- low, raised, with undulate margin. Gelatin stab: No liquefaction (Schroeter, in Cohn, Krj^ptog. Flora v. Schlesien, 3, I, 1886, 144). Agar colonies: Small, yellowish, glisten- ing, raised. Agar slant: Citron-yellow, smooth growth. Broth: Clear, with yellowish sediment. Litmus milk: Usually slightly acid, not coagulated. Potato: Thin, glistening, citron-yellow growth. Indole not produced. Acid from glucose, sucrose and mannitol. No acid from lactose. Starch not hydrolyzed. Nitrites not produced from nitrates. Ammonia produced from peptone. Utilizes NH4H2PO4 as a source of nitro- gen; also utilizes ammonium tartrate, growth occurring after 6 weeks in Cohn's solution (Cohn, op. cit., 1872, 153). Saprophytic. Aerobic. Optimum temperature, 25° C. (Eisenberg, Bakt. Diag., 1891, 39). Comments: It is recommended that Cul- ture number 398, Micrococcus luteus, of the American Type Culture Collection be ac- cepted as the type culture for this species. This culture has been retested (September, 1955) and has been found to grow slowly in Cohn's solution without added sugar after 6 weeks under conditions similar to those used by Cohn in 1872. On reinoculation, this culture now develops within 2 weeks in Cohn's solution. Source: Isolated by Schroeter from dust contaminations on cooked potato. Habitat: Found in milk and dairy pro- ducts and on dust particles. 2. Micrococcus ureae Cohn, 1872. (Bei- triige z. Biol. d. Pflanzen, 1, Heft 2, 1872, 158.) u're.ae. Gr. noun ururn urine; M.L. noun tirea urea; M.L. gen. noun ureae of urea. Spheres, 0.8 to 1.0 micron in diameter, oc- curring singly, in pairs and in clumps. Never in chains. Non-motile. Gram-variable. Gelatin colonies: Small, white, translu- cent, slimy, becoming fissured. Gelatin stab: Slight, white growth. Very slow or no liquefaction. Agar colonies: White, slightly raised. Agar slant: Grayish white, raised, glisten- ing, butyrous growth. Broth: Turbid, with viscid sediment. Litmus milk: Slightly alkaline; litmus slowly reduced. Milk: Acid. Potato: Slight, grayish to pale olive growth. Indole not produced. Acid produced from glucose, lactose, sucrose and mannitol. Starch not hydrolyzed. Nitrites not produced from nitrates. Urea fermented to ammonium carbonate. Ammonium salts are utilized. Ammonia produced from peptone. Saprophytic. Aerobic. Optimum temperature, 25° C. Source: Isolated from fermenting urine. Habitat : Found in stale urine and in soil containing urine. 3. Micrococcus freudenreichii Guille- beau, 1891. (Landwirtsch. Jahrb. d. Schweiz, 5, 1891, 135.) freud.en.reich'i.i. M.L. gen. noun freu- denreichii of Freudenreich; named for E. von Freudenreich, a Swiss bacteriologist. Spheres, 2.0 microns in diameter, occur- ring singly and in clumps, rarely in short chains. Non-motile. Gram-positive. Milk gelatin colonies: Small, white, opaque. Milk gelatin stab : Infundibuliform lique- faction. Agar colonies: White, slimy. Agar streak: White, smooth growth. Broth: Turbid, with white sediment.