Page:Bergey's manual of determinative bacteriology.djvu/471

 perianal abscess and two strains from puru- lent meningitis in man. Habitat: Found in various infections in man. 14. Sphaerophorus floccosus (Wein- berg et al., 1937) Prevot, 1938. (Strepfobadl- hts pyogenes floccosus Courmont and Cade, Arch. Med. Exp., 12, 1900, 393; Bacillus floccosus Weinberg et al., Les Microbes Ana6robies, 1937, 698; not Bacillus floccosus Kern, Arb. bakt. Inst. Karlsruhe, 1, Heft 4, 1896, 424; Bacteroides floccosus Hauduroy et al., Diet. d. Bact. Path., 1937, 55; Prevot, Ann. Inst. Past., 60, 1938, 299.) floc.co'sus. L. adj. floccosus full of flocks of wool. Small, ellipsoidal rods, 1 micron long, oc- curring singly, in pairs and in chains. Non- motile. Show bipolar staining. Gram-nega- tive. Gelatin: No liquefaction. Deep agar: Growth slow, appearing as a light haze. No gas produced. Blood agar: Translucent colonies sur- rounded by a zone of alpha hemolysis. Broth: Rapid, flocculent growth on sides of tube; no turbidity; no gas; slight fetid odor. Milk: Poor growth; no coagulation. Coagulated serum: Small, whitish colo- nies. No liquefaction. Potato: No growth. Anaerobic. Optimum temperature, 37° C. Non-pathogenic for mice. Produces le- sions in rabbits, guinea pigs and dogs. Source: Isolated from blood in pyemia of man. Habitat: Presumabh' found in various in- fections in man. 15. Sphaerophoru.s abscedens Tardieu.x and Monteverde, 1951. (Tardieux and Monteverde, in Tardieux, Ann. Inst. Past., 80, 1951,276.) ab.sce'dens. L. part. adj. abscedens going away, disappearing. Pleomorphic rods which, on initial cultur- ing, occur as ellipsoidal forms, 2 to 3 microns in diameter, and as elongated forms with lateral or terminal, spheroidal swellings and metachromatic granules. With subse- quent transfers, the short forms predomi- nate, especially in liquid media, and pleo- morphism is retained in agar stabs. The ellipsoidal forms show bipolar staining. Non-motile. Gram-negative. Serum or ascitic fluid is required for growth. Neither gas nor odor produced. Gelatin: No liquefaction. Serum agar stab: Colonies punctiform, becoming lenticular. Glucose serum broth: Homogeneous tur- bidity. In glucose broth, the end-products of fer- mentation are acetic, butyric and lactic acids, ammonia, a slight amount of hydro- gen sulfide, indole and sometimes cresol and acetylmethylcarbinol. Amines, alcohols and ketones are not produced. Peptone broth: Eventually becomes tur- bid; indole is produced. Milk: Unchanged. Coagulated proteins not attacked. Glucose and galactose are attacked. Nitrites not produced from nitrates. Anaerobic. Neither toxin nor hemolysin produced. Non-pathogenic for rabbits, guinea pigs or mice. Source: One strain was isolated from the pus of an abscess on a cow; a second strain was isolated from the pus of an abscess which developed on the heel of a man bitten by a dog. Habitat : Found in warm-blooded animals so far as known. 16. Sphaerophorus caviae (Vinzent, 1928) Prevot, 1938. iStreptobacillus caviae Vinzent, Ann. Inst. Past., 42, 1928, 533; Bacteroides caviae Hauduroy et al.. Diet. d. Bact. Path., 1937, 53; Prevot, Ann. Inst. Past., 60, 1938,299.) ca'vi.ae. M.L. gen. noun caviae of Cavia; M.L. noun Cavia generic name of the cavy, or guinea pig. Small rods, usually 0.3 to 0.5 by 1.0 to 1.5 microns, sometimes curved, occurring singly and in chains. Pleomorphic in old cultures with long, filamentous forms. Non-motile. Gram-negative. Serum or ascitic fluid is required for growth.