Page:Bergey's manual of determinative bacteriology.djvu/444

 slightly cloudy growth; sticky, grayish sediment at the bottom of the tube in old cultures. Acid from glucose, fructose, mannose, sucrose, galactose, maltose, salicin, xylose, mannitol, dextrin, arabinose, amygdalin and lactose. Small amount of acid from raffinose, inulin, rhamnose and trehalose. No acid from dulcitol, sorbitol or inositol. Aerobic, facultatively anaerobic. Temperature relations: Optimum, be- tween 15° and 30° C. Grows at 37° C. Optimum pH, 7.8. Non-pathogenic for rabbits, guinea pigs, rats and mice. Distinctive characters: Non-motile when cultured on Leptospira medium or on blood agar plates at 30° to 37° C; motile when grown on horse blood agar slants at 30° C. or on Leptospira medium or blood agar plates at 15° C. Carbohydrates are attacked. Agglutination reactions. Source : Isolated from cases of trachoma of American Indians at Albuquerque, New Mexico. Habitat: Regarded by Noguchi and by others as a cause of trachoma in man. Pro- duces a granular conjunctivitis in monkej^s and apes. 2. Noguchiasimiae (Olitsky et al., 1933) Olitsky et al., 1934. (Bacterium simiae Olitsky, Syverton and Tyler, Jour. Exp. Med., 57, 1933, 875; Olitsky, Syverton and Tyler, ibid., 60, 1934, 382.) si'mi.ae. L. noun simia ape; L. gen. noun simiae of an ape. Slender rods, 0.2 to 0.3 by 0.8 to 1.2 mi- crons, with pointed ends, occurring singly, in pairs, in short chains or in a parallel arrangement of two or three cells. Encapsu- lated. Motile by means of flagella which are really of the peritrichous type, although normally not more than one flagellum is found attached near the end but usually not in the polar position; occasionally two separated flagella are observed in lateral positions. Gram-negative. Gelatin colonies: More mucoid and more raised than those on agar. Gelatin stab: Arachnoid growth along line of inoculation. No liquefaction. Agar colonies: Small, circular, grajdsh, translucent, smooth, convex, slightly raised with a sticky or mucoid consistency. Agar slants: Grayish white to white, moist, mucoid, raised, glistening growth. Growth is more profuse when blood is added. Blood agar colonies : More highly translu- cent and colorless during earlj- growth than those on plain agar, becoming grayish after two or three daj's. Leptospira medium: Homogeneous, dense growth in a 0.5 cm, sharply defined layer with a slight, nebulous, uniform opacity about 1 cm below. In three or four days the lower layer becomes more dense and in time extends to the bottom of the tube. Broth: Uniformly turbid; slight, grayish sediment; no pellicle. Litmus milk: Unchanged. Potato: Light tan, spreading, abundant growth. Indole not produced. Acid but no gas from glucose, fructose, mannose, galactose, xylose, arabinose and rhamnose. Small amount of acid from dex- trin. Some strains produce a small amount of acid from sucrose, lactose, inulin and mannitol. Raffinose, salicin, dulcitol, amyg- dalin, maltose, trehalose, sorbitol and inosi- tol are not attacked. Nitrites not produced from nitrates. Aerobic, facultatively anaerobic. Temperature relations: Optimum, be- tween 28° and 30° C. Thermal death point, 56° C. for thirty minutes. Serological reactions: Rabbit antiserum is specific for all strains, and there is no cross agglutination with Noguchia granulo- sis. Distinctive characters: Attacks carbohy- drates. Agglutination reactions. Source: Isolated from inflammatory type, Type II, of spontaneous conjunctival folliculosis in monkeys (Macacus rhesus). Habitat: Causes conjunctival folliculosis in monkeys (Macacus rhesus). 3. Noguchia cuniculi Olitsky et al., 1934. (Olitsky, Syverton and Tyler, Jour. Exp. Med., 60, 1934, 382.) cu.ni'cu.li. L. noun cunioilns rabbit; L. gen. noun cuniculi of a rabbit.