Page:Bergey's manual of determinative bacteriology.djvu/379

 a'na.nas. M.L. noun Ananas generic name of the pineapple. Note: Not to be confused with Pseudo- 7nonas (Phytomonas) ananas Serrano, loc. cit. Short rods, 0.6 bj' 0.9 micron, with rounded ends, occurring singly, in pairs and in short chains. Motile by means of peri- trichous flagella. Encapsulated. Gram-nega- tive. Gelatin stab: Stratiform liquefaction with a deep, chrome-yellow sediment. Potato glucose agar: After 24 hours, circu- lar, 3 mm in diameter, convex, dense, ho- mogeneous, entire, moist, straw-yellow, mottled, becoming primulin-yellow. Plates have a molasses odor. Show two types of colonies: rough and smooth. Rough colonies have crenate margins. Potato glucose agar slant: Growth straw- yellow, raised, becoming primulin-yellow, moist, glistening. Broth: Turbid; straw-colored pellicle and ring. Glucose broth: Growth sulfur-yellow. Litmus milk: Coagulated, faintly acid, be- coming alkaline. Potato: Copious growth, moist, glisten- ing, spreading, becoming primulin-yellow. Indole not produced. Slight amount of hydrogen sulfide pro- duced. Blood serum: Moderate growth, slightly raised, mustard-yellow to primulin-yellow. No liquefaction after 3 months. Cohn's solution: No growth. Phenol-negative. Diastase-positive. No gas from carbohydrates. Acid from glucose, lactose, sucrose, mannitol, raffi- nose, glycerol, salicin, dextrin, maltose, fructose and mannose. No acid from arabi- nose, xylose, amygdalin, rhamnose, inositol, inulin, dulcitol, adonitol, asparagine or starch. Small amount of alcohol and aldehyde produced. Nitrites produced from nitrates. Slight amount of ammonia produced. Source: Isolated from the pineapple {Ana- nas sativus) and sugar cane (Saccharian offi- cinarum). Habitat : Causes a brown rot of the fruit- lets of pineapple. 14. Erwinia aroideae (Townsend, 1904) Holland, 1920. (Bacillus aroideae Townsend, U. S. Dept. Agr., Bur. Plant Ind. Bull. 60, 1904, 40; Holland, Jour. Bact., 5, 1920, 222; Pectobacterinm aroideae Waldee, Iowa State Coll. Jour. Sci., 19, 1945, 472.) a.ro.i'de.ae. Gr. noun arum the plant wake robin; M.L. pi. noun Aroideae the name of the Arum subfamily; M.L. gen. noun aroideae of an aroid. Description taken from Townsend (op. cit., 1904, 40) and supplemented by studies made by Burkholder. Rods, 0.5 by 2.0 to 3.0 microns; occasion- ally a few very small rods occur. Motile by means of 2 to 8 peritrichous flagella. Gram- negative. Gelatin: Liquefaction. Beef-peptone agar slants: Light to mod- erate growth, filiform, white to cream. Broth: Turbid; no pellicle. Litmus milk: Coagulation in 3 days. Lit- mus reduced. One-fourth of tube whey. Potato plug: White with tinge of yellow. Endo agar slants: Streak deep red and medium deep red. Krumwiede's triple sugar agar: Turns yel- low but later a red color appears at top of slant. Fermi's solution: Flocculent and white. Uschinsky's solution: Very turbid with heavy sediment. Desoxycholate agar: Pink, later yellow- ish. Indole not produced. Hydrogen sulfide produced. Acid but no gas from glucose, galactose, fructose, arabinose, xylose, lactose, sucrose, glycerol, mannitol and salicin. Maltose doubtful. Alkaline reaction in sodium cit- rate, and only slight growth in tartrate in 10 days. Methyl red test weakly positive; acetj'l- methylcarbinol produced. No growth in ethanol, dulcitol, sodium hippurate and malonate. Starch not hydrolyzed; ammonium pec- tate medium liquefied. Nitrites rapidly produced from nitrates. Good growth in 5 per cent salt, and a de- layed but good growth in 7 per cent salt. Aerobic.