Page:Bergey's manual of determinative bacteriology.djvu/348

 sline, 1933) Breed, comb. nov. {Achromobac- ter pastinator Goresline, Jour. Bact., 26, 1933, 442.) pas.ti.na'tor. L. noun pastinator one who digs or trenches the ground. Short rods, 0.4 by 1.5 microns, occurring singly and in pairs. Motile by means of two to five peritrichous flagella. Gram-negative. Plain gelatin stab: No growth. Nutrient gelatin stab: Surface growth very scant. No liquefaction. Nutrient agar colonies: At first tiny, almost colorless, becoming yellowish and ring-like. Agar liquefied rapidly. Nutrient agar slant: Growth good, flat not thick. Agar liquefied along streak often to the depth of a quarter of an inch. Pocket formed at bottom of slant filled with a rather viscous, yellowish fluid. Nutrient broth: Slight turbidity after 5 days. Subsurface but no surface growth. No sediment. Litmus milk: Slightly acid after 20 days. No curd. Only a trace of reduction at bot- tom of tube. Potato: No growth. Indole not produced. Hydrogen sulfide not produced. Acid from glucose, fructose, arabinose, galactose, sucrose, maltose, lactose, man- nose, melezitose, raffinose, rhamnose, sali- cin, pectin, starch and dextrin. No growth in dulcitol, erythritol, mannitol, sorbitol, glycerol, xylose or inulin. Starch is hydrolyzed. Agar is digested. Temperature relations: Optimum, 28° C. Good growth at 25° C. Moderate growth at 20° and at 37° C. No growth at 10° or at 42° C. Limits of growth: pH 5.9 to 9.0. Facultative anaerobe. Distinctive characters: Digests agar rapidl}^; colonies sink through to the glass of the Petri dish. Fehling's solution is re- duced by the liquefied agar. There is con- siderable change in the viscosity of the agar due to the digestion. Source: Isolated from a trickling filter receiving creamery wastes. Habitat: Probably widely distributed in nature. 7. Agarbacteriuiti uliginosuni (ZoBell and Upham, 1944) Breed, comb. nov. {Flavo- bacterium idiginosum ZoBell and Upham, Bull. Scripps Inst, of Oceanography, Univ. Calif., 5, 1944, 263.) u.li.gi.no'sum. L. adj. uliginosus wet, damp. Rods, 0.4 to 0.6 by 1.2 to 3.9 microns, some slightl}' curved, occurring mostly singly with some short chains. Non-motile. Gram-negative. All difi'erential media except the fresh- water broth, litmus milk and potato were prepared with sea water. Gelatin colonies: 1 mm in diameter, orange, sunken. Gelatin stab: Infundibuliform liquefac- tion. Yellow pigment. Gelatin discolored brown. Agar colonies: Sunken, uneven, irregular, gummy; agar is liquefied. Orange to yellow pigment is produced, and agar is discolored brown. Agar slant: Luxuriant, yellowish orange, glistening, filiform, adherent growth; agar slowly liquefied. Sea-water broth: Moderately turbid; dense, j^ellow pellicle; slightly viscid sedi- ment. Fresh-water broth: No visible growth. Litmus milk: Completely decolorized; neutral. Potato: No visible growth. Indole not produced. Hydrogen sulfide not produced. Acid but no gas from xjdose, glucose, maltose, lactose, sucrose and salicin. Glyc- erol and mannitol not fermented. Starch not hydrolyzed. Agar rapidly liquefied. However, after prolonged laboratory cultivation, this or- ganism gradually loses its ability to digest agar. Nitrites rapidly produced from nitrates. Ammonia produced from peptone but not from urea. Casein digested. Non-lipolj'tic. Aerobic, obligate. Optimum temperature, between 20° and 25° C. Source: Isolated from marine bottom de- posits.