Page:Bergey's manual of determinative bacteriology.djvu/347

 Source: Isolated from fronds of Nereocystis luetkeana.

Habitat: Found on marine algae.

4. Agarbacterium = reducans Angst, 1929. (Puget Sound Biol. Sta. Pub., 7, 1929, 57.)

re.du’eans. L. part. adj. reducans bring- ing back, reducing.

Short rods, 0.6 by 0.8 with rounded ends, occurring singly and in pairs. Not encapsulated. Motile. Gram-negative.

All media contained a decoction made by boiling or autoclaving fronds of Iridaea, a seaweed, in sea water or a 3 per cent salt solution.

Fish gelatin colonies: Circular, sunken, entire, crateriform, granular; growth is rapid.

Fish gelatin slant: Abundant, filiform, sunken, glistening, smooth, opaque, buff, butyrous growth.

Fish gelatin stab: Crateriform liquefac- tion; growth best at top.

Agar colonies: Circular, smooth, flat, entire, granular; growth is moderate.

Agar slant: Abundant, filiform, flat, glis- tening, smooth, opaque, buff, butyrous growth.

Fish agar slant: Abundant, filiform, flat, glistening, smooth, opaque, white, butyrous growth.

Meat agar slant: Abundant, echinulate, flat, glistening, smooth, opaque, white, butyrous growth.

Broth: Turbid, no sediment; no pellicle.

Plain milk unchanged.

Potato: No growth.

Indole not produced.

Hydrogen sulfide not

Acid from sucrose, rhamnose, arabinose and mannitol. No acid from lactose or xylose.

Starch is hydrolyzed.

Agar is digested; cellulose not attacked.

Aerobic.

Methylene blue reduced.

Temperature relations: Optimum, be- tween 25° and 28° C. Minimum, less than 5° C. Maximum, between 36° and 45° C.

Source: Isolated from Nereocystis luet- keana.

Habitat: Found on marine algae.

5. Agarbacterium amocontactum (Zo- Bell and Allen, 1935) Breed, comb. nov. (Flavobactertum amocontactum ZoBell and Allen, Jour. Bact., 29, 1935, 246.)

a.mo.con.tac’tum. L. v. amo to like, love; L. noun contactus contact; M.L. adj. amo- contactus presumably means contact-loving.

Slender rods, 0.4 to 0.7 by 1.6 to 2.3 mi- crons, with rounded ends, occurring singly and in irregular clumps. Encapsulated. Actively motile by means of peritrichous flagella. Stain very lightly. Gram-negative.

Gelatin stab: Good, filiform growth with rapid, saccate liquefaction.

Agar colonies: Circular, 2 to 4 mm in di- ameter, yellow.

Agar slant: Abundant, filiform, smooth, glistening, bright yellow growth with a butyrous consistency. Originally agar was liquefied, but this property was lost follow- ing artificial cultivation.

Sea-water broth: Good growth with ring at surface. Strongly turbid; abundant, vis- cid sediment; no odor.

Milk: No growth.

Potato: No growth.

Potato dialyzed in sea water: Slight, yel- low growth.

Indole not produced.

Hydrogen sulfide produced.

No acid from glucose, lactose, sucrose, xylose or mannitol.

Starch not hydrolyzed.

Agar is digested.

Nitrites produced from nitrates.

Ammonia produced from peptone.

Aerobic, facultatively anacrobic.

Optimum temperature, between 18° and 21° C.

Optimum reaction, pH 8.0.

Distinctive character: Adheres firmly to submerged glass slides and cannot be re- moved with running water.

Source: Many cultures were isolated from glass slides submerged in sea water.

Habitat: Sea water.

6. Agarbacterium pastinator (Gore-