Page:Bergey's manual of determinative bacteriology.djvu/338

 Broth: Turbid with white sediment. Blood serum: Light graj^-colored growth in 2 to 3 days. Litmus milk: Action not recorded. Potato: No growth. Aerobic, facultatively anaerobic. Optimum temperature, 35° C. Pathogenic for laboratory animals. Source : Found constantly in Berlin drain water. Habitat: Sewage. 11. Flavobacterium halmephiluin Ela- zari-Volcani, 1940. (Studies on the Micro- flora of the Dead Sea. Thesis. Hebrew Univ., Jerusalem, 1940, VIII and 85.) hal.me'phi.lum. Gr. noun halme brine, sea water; Gr. adj. philus loving; M.L. adj. halinephilus sea-water-loving. Rods, 0.5 to 0.6 by 0.7 to 2.0 microns, occurring singly and in pairs; morphology and size unchanged as salt concentrations vary. Non-motile. Gram-negative. Gelatin stab (12 per cent salt-1 per cent proteose peptone-15 per cent gelatin) : Scant growth; no liquefaction after a month. Agar colonies (12 per cent salt-1 per cent proteose peptone-2 per cent KNO3) : Circu- lar, smooth, entire, convex, glistening, opaque, yellowish. Agar slant (12 per cent salt-1 per cent proteose peptone-2 per cent KNO3) : Moder- ate, hliform, raised, smooth or slightly rugose, opaque, j^ellowish growth. Broth (12 per cent salt-1 per cent pep- tone) : Turbid; delicate pellicle or ring and granular, flaky sediment; broth turns yellowish. Indole not produced. No acid or gas from glucose, fructose, galactose, mannose, lactose, sucrose, mal- tose, arabinose, xylose, raffinose, inulin, dextrin, glycerol, mannitol or salicin. Starch not hydrolyzed. Nitrites produced from nitrates in trace amounts. Aerobic. Optimum temperature, 30° C. Salt tolerance: Halotolerant, not grow- ing in 0.5 per cent salt, but growing strongly in 3 to 24 per cent and moderately in 30 per cent salt and in Dead Sea water. Source: Isolated from the water of the Dead Sea. Habitat: Found in places where the salt content of water is high. 12. Flavobacteriiuii rhenaniim (Mi- gula, 1900) Bergey et al., 1948. (Burri's Rhine water bacillus, Frankland and Frank- land, Microorganisms in Water, 1894, 483; Bacillus rhenanus Migula, Syst. d. Bakt., 2, 1900, 713; Flavobacterium rhenanus (sic) Bergey et al.. Manual, 6th ed., 1948, 433.) rhe.na'num. L. adj. rhenanus pertaining to the Rhine. Original description supplemented by Bergey {loc. cit.) from his private notes as indicated; Steinhaus (Jour. Bact., ^2, 1941, 762 and 772) apparently found the same organism and has added other characters. Rods, 0.7 by 2.5 to 3.5 microns, with rounded ends, occurring singly and in chains (Burri). Motile, possessing peritrichous flagella (Bergey). Gram-negative (Bergey). Gelatin colonies: Convex, colorless, transparent, becoming yellowish. Gelatin stab: Infundibuliform liquefac- tion. Agar colonies: Small, smooth, convex, entire. Agar slant : Bright yellow growth (Stein- haus) . Glycerol agar slant: Thin, shining, honey- colored. Growth dry and tough. Broth: Turbid, with orange-colored pel- licle and sediment. Litmus milk: Soft coagulum, becoming slightly alkaline with yellow ring. Potato: Moist, glistening, thin, flat, orange to rust-colored growth. Indole not produced (Bergey) . Hydrogen sulfide not produced (Stein- haus) . Acid from glucose, maltose and sucrose but not from lactose (Steinhaus). Starch not hydrolyzed (Steinhaus) . Nitrites produced from nitrates (Bergey). Aerobic, facultatively anaerobic. Optimum temperature, 30° C. Source : Isolated from Rhine River water (Burri). From water (Bergey). From eggs in the ovary of a walking stick {Diaphero- mera femorata Say) (Steinhaus).