Page:Bergey's manual of determinative bacteriology.djvu/337

 1895, 442; Bergey et al., Manual, 1st ed., 1923, 115.) dor.mi.ta'tor. L. noun dunnitator a sleeper, a sluggard. Original description supplemented by material taken from Harrison (Canadian Jour, of Research, 1, 1929, 233), whose cul- tures differed in some particulars from Wright's. Rods with conical ends, occurring singly, in pairs and in chains. Non-motile. Gram- negative (Harrison). Gelatin colonies: Small, yellow, slightly granular. Liquefaction. Gelatin stab: Infundibuliform liquefac- tion; yellow sediment. Agar slant: Yellow, glistening, translu- cent growth. Ammonium phosphate agar: Slight, yel- low growth. Broth: Turbid with slight pellicle and yellow sediment. Litmus milk: Slightly acid; litmus re- duced. Harrison reports no reduction. Potato: Slight, transparent, yellow growth. Indole not produced (Harrison) . Acid from glucose, sucrose, glycerol and mannitol. Xo acid from lactose, rafRnose or inulin (Harrison). Nitrites produced from nitrates in trace amounts (Harrison). Aerobic, facultatively anaerobic. Optimum temperature, 30° C. Source: Originally isolated from fresh water at Philadelphia. Later isolated by Harrison {loc. cit.) from the skin of halibut taken from the Pacific Ocean off Canada. Gibbons (Contrib. to Canadian Biol, and Fish., 8, 1934, 279) reports this species as occurring in the slime of a haddock {Mel- anogrammus aeglefinus). Habitat: Found in fresh and salt water. 9. Flavobacteriuin peregriniim Stapp and Spicher, 1954. (Zent. f. Bakt., II Abt., 108, 1954, 113.) per.e.gri'num. L. adj. peregrimis strange, foreign. Straight rods, 0.5 to 0.8 by 2.4 microns, occurring singly and in pairs. Non-motile. Gram-negative. Gelatin colonies: Circular, entire, j^ellow; radial grooves. Gelatin stab: Yellow surface growth; no growth in the stab. No liquefaction. Nutrient agar colonies: Mucoid, yellow, shiny; hyaline margins. Glucose agar: Slowly developing yellow growth. Nutrient broth: Turbid with a white sediment. Litmus milk: Unchanged. Potato: Yellow streak. Indole not produced. No acid or gas from glucose, sucrose, lactose, glycerol or mannitol in nutrient broth. Starch not hydrolj-zed. Nitrites not produced from nitrates. Slow development in a mineral, glucose agar. Aerobic. Distinctive characters: Resembles Flavo- baclerium breve Bergey et al. culturally. The herbicide 2,4-dichlorophenoxyacetic acid is utilized as a sole source of carbon in an otherwise inorganic medium. Destroys 2,4-D in the soil, presumably by opening the benzene ring. Source: Isolated in Germany from soil- enrichment cultures containing 2,4-D. Habitat: Found in soil. 10. Flavobacteriuni breve (Lustig, 1890) Bergey et al., 1923. (Der kurze Canalbacil- lus, Mori, Ztschr. f. Hyg., 4, 1888, 53; Bacillus canalis parvus Eisenberg, Bakt. Diag., 1891, 362; Bacillus brevis Lustig, Diagnostica dei batteri delle acque, Torino, 1890, 52; Bacterium canale Mez, Mikro- skopische Wasseranalyse, Berlin, 1898, 55; Flavobacteriuni brevis (sic) Bergey et al., Manual, 1st ed., 1923, 116.) bre've. L. adj. brevis short. Rods, 0.8 to 1.0 by 2.5 microns, showing polar staining. Non-motile. Gram-negative. Gelatin colonies: Minute, pale yellow, compact growth in 2 to 3 weeks. Gelatin stab: Thin, j-ellowish growth on the surface in 3 weeks. Beaded growth in stab. No liquefaction. Agar slant: Yellowish growth in 2 to 3 days.