Page:Bergey's manual of determinative bacteriology.djvu/264

 rounded ends, occurring singly, in pairs, and at times in short chains. Motile. Polar flagellate (Stanier, loc. cit.). Gram-negative. Fish-gelatin colonies: Punctiform, black, glistening. Fish-gelatin stab: Slow, crateriform lique- faction. Sea-weed agar colonies: Circular, flat, opaque, glistening, white, slimy, entire. Agar is dissolved. Fish-agar slant: Flat, white, elevated, glistening, undulate growth. Liquefaction. Broth: Turbid with grayish white, slimy sediment. Indole not produced. No action on sugars. Starch usuallj^ hydrolyzed. Nitrites not produced from nitrates. Aerobic, facultative. Optimum temperature, between 20° and 25° C. Minimum, between 0° and 5° C. Max- imum, between 30° and 32° C. Source: Isolated from sea-water of the Norwegian Coast. Habitat: Presumably found in sea water and on sea weeds.

21. Vibrio neocistes Gray and Thorn- ton, 1928. (Cent. f. Bakt., II Abt., 73, 1928, 92.) ne.o.cis'tes. Gr. adj. nensnev;; Gr. noun ciste box; M.L. fern. gen. n. neocistes of New- ark, a city. Curved rods 0.5 to 1.0 by 1.0 to 4.0 mi- crons. Motile by means of one to three polar flagella. Gram stain not recorded. Gelatin colonies: Liquefied. Gelatin stab: Liquefied. Medium red- dened. Agar colonies: Circular or amoeboid, buff to brownish, convex, smooth, glistening, entire. Agar slant: Filiform, fluorescent, raised, smooth, glistening, undulate. Broth: Turbid. Acid from glucose. Starch not hydrolyzed. Nitrites not produced from nitrates. Attacks naphthalene. Aerobic, facultative. (Optimum temperature, between 30° and 35° C. Habitat: Soil.

22. Vibrio cyclosites Gray and Thorn- ton, 1928. (Cent. f. Bakt., II Abt., 73, 1928, 92.) cyc.lo.si'tes. Gr. nonn ajclus a ring: Gr. V. sited to eat; M.L. adj. cyclosites feeding on rings, i.e., on ring compounds. Curved rods 0.5 to 1.0 by 1.5 to 4.0 mi- crons. Motile by means of a single, polar flagellum. Gram-negative. Gelatin colonies: Circular, buff to brown, flat, smooth, glistening, entire. Gelatin stab: No liquefaction. Agar colonies: Circular to irregular, pale buff (later greenish), smooth, entire. Agar stab: Filiform, greenish buff, raised, smooth, undulate. Broth: Turbid. Indole not reported. Acid from glucose. Starch not hydrolj'zed. Nitrites not produced from nitrates. Attacks phenol and ?«-cresol. Aerobic, facultative. Optimum temperature, between 30° and 35° C. Habitat: Soil.

23. Vibrio oxaliticus Bhat and Barker, 1948. (Jour. Bact., 55, 1948, 359.) ox.a.li'ti .cus. Gr. noun oxalis sorrel, a sour plant; Gr. adj. lyticus dissolving; M.L. adj. oxaliticus intended to mean decom- posing oxalate. Curved rods 0.4 by 1.3 microns. Actively motile by means of a single, polar flagellum. Not encapsulated. Gram-negative. Nutrient agar colonies: Small, moist, raised, entire; no chromogenesis. Pin-point in size in 48 hours, growing slowly until they reach a diameter of 1.5 mm in 6 days. Nutrient broth: Moderate growth after 24 hours, appearing at first as a thin film while a slight, general turbidity develops in another 24 to 48 hours. Calcium oxalate agar: Growth rapid and colonies small; medium becomes alkaline. Oxalate broth: Becomes turbid following the formation of a slight surface film. Oxalates and pyruvates support growth within 3 to 4 days when added to a mineral medium as the sole carbon source; formates support growth only when the incubation period is extended. The following do not