Page:Bergey's manual of determinative bacteriology.djvu/259

 (Roy. Soc. Queensland, Proc. for 1937, 49, 1938, 29.) ha.lo.ni.tri'fi.cans. Gr. noun hals, hairs the sea salt; M.L. part. adj. nitrificans ni- trifying; M.L. adj. halonitrificans nitrifying salt. Curved rods, usually 0.3 by 1.2 to 2.5 mi- crons, occurring singly. Motile by means of a single, polar flagellum. No marked varia- tion in form in media of varied salinity. Stain somewhat faintly with the usual stains. Gram-negative. No growth on media which does not con- tain salt. Limit for growth, 1 to 23 per cent salt; optimum growth in 4.0 to 6.0 per cent salt. Gelatin stab: Liquefied within 7 days at 35° C.; at 20° C. shallow, superficial lique- faction was evident in 20 days. Agar colonies: Light amber, glistening, convex, transparent, non-viscid, slightly spreading. Agar slants: Growth slow at 4° C., col- onies appearing in 14 days. Nutrient and nitrate broths: Growth. No growth, however, when covered with a paraffin oil seal. Litmus milk: Not coagulated; growth slight or absent. Potato: Growth moist, fairly abundant, whitish. Indole not produced. Hydrogen sulfide not produced. Glucose, fructose, sucrose, mannose, rhamnose, galactose, lactose, maltose, raf- finose, sorbitol and glycerol not fermented. Acetylmethylcarbinol not produced. Non-lipolytic. Nitrites produced from nitrates. Catalase-negative. Aerobic. Temperature relations: Optimum, be- tween 30° and 35° C. Slow growth at 4° C. Killed in 10 minutes in 6 per cent saline broth at 55° C. Limiting reactions for growth, pH 5.4 and pH 9.2. Not pathogenic for guinea pigs or mice. Source: Five strains were isolated from tank brines from bacon-curing factories in Australia. The strains showed little var- iation in characters. Except for its ability to liquefy gelatin, this species resembles the organisms isolated by Sturges and Heideman (Absts. Bact., 7, 1923, 11; ibid., 8, 1924, 14; ibid., 9, 1925, 2) in the U. S. A. Habitat: Known to be found in meat- curing brines but probably more widely distributed.

12. Vibrio luminosus Beijerinck, 1888. (Vibrio luminosus (nomen nudtim) Beije- rinck, Botan. Zeitg., 46, 1888, 763; Photo- bacterium luminosum Beijerinck, Arch. N^erl. d. Sci. Exact, et Natur., 23, 1889, 401 ; Microspira luminosa Migula, Syst. d. Bakt., 2, 1900, 1015.) lu.mi.no'sus. L. adj. luminosus Inminons. Small rods having the size and form of the cholera vibrio when grown in media con- taining little nitrogen and carbohydrates. Sometimes form chains of vibrios which resemble spirilla. In richer media the cells become much larger. Motile. Gram-nega- tive (Chester, 1897). Gelatin: Liquefied. In presence of 0.5 per cent asparagine and 0.5 per cent peptone, offensive odors not produced. Putrefaction of the gelatin occurs when the nitrogen source is insufficient. Peptonized meat bouillon gelatin: No growth. Good growth and luminescence with the addition of 3.0 to 3.5 per cent of sea salt, potassium chloride or magnesium chloride. Agar: Growth rapid, shines feebly. Sea-water broth: Produces forms which resemble the bacteroids of legume bacteria. Blood serum: No growth. Addition of 3.0 to 3.5 per cent of sea salt, potassium chloride or magnesium chloride allows good growth and luminescence. Nitrates not reduced. Indigo-blue not readily reduced. Salt tolerance: In order to assure phos- phorescence and good growth, the osmotic tension of inorganic salt solutions used for cultivation should be equivalent to that produced in a 3 per cent sodium chloride solution. Optimum temperature for growth and luminescence, between 25° and 28° C. Aerobic. Quality of luminescence: Bluish green, persisting for 1 to 2 weeks. Distinctive characters: Develops only