Page:Bergey's manual of determinative bacteriology.djvu/183

. Litmus reduced. Crystal formation (Burkholder).

Nitrites not produced from nitrates.

Nitrogen sources utilized are peptone, aspartic acid, alanine, leucine, sodium ammonium phosphate, allantoin, tyrosine, uric acid and brucine.

Indole not produced.

Hydrogen sulfide not produced on lead acetate agar. H2S produced after ZoBell and Feltham's method (Burkholder).

Selenium dioxide reduced.

Lipolytic (Starr and Burkholder, ibid., 600).

Acid but no gas from glucose, fructose, galactose, lactose, sucrose, maltose, xylose, raffinose, mannitol, glycerol and starch. Alkali from salts of citric, lactic, malic and succinic acids. Arabinose, rhamnose, dulcitol, salicin, inulin and cellulose not utilized.

Starch hydrolyzed.

Pectate medium not liquefied.

Temperature relations: Optimum, between 28° and 32° C. Minimum, between 5° and 7° C. Maximum, 37° C. Thermal death point between 53° and 55° C.

pH range for growth: pH 5.2 to 10.5. Optimum pH, between 6 and 8.

Strict aerobe.

Distinctive characters: Cultural characters the same or similar to those of Xanthomonas juglandis. The two species do not cross-infect.

Source: 26 isolates from widely scattered filbert orchards in Oregon and Washington.

Habitat: Pathogenic on filberts (Corylus avellana and C. maxima).

15. Xanthomonas cucurbitae (Bryan, 1926) Dowson, 1939. (Bacterium cucurbitae Bryan, Science, 63, 1926, 165; Bryan, Jour. Agr. Res., 40, 1930, 389; Dowson, Zent. f. Bakt., II Abt., 100, 1939, 190.)

cu.cur'bi.tae. L. cucurbita a gourd; M.L. fem.n. Cucurbita generic name; M.L. gen.noun cucurbitae of Cucurbita.

Rods 0.45 to 0.6 by 0.5 to 1.3 microns. Motile, usually with a single polar flagellum. Gram-negative.

Gelatin: Liquefied.

Beef-agar slants: Growth moderate, mustard-yellow, undulating margins, viscid to butyrous.

Broth: Moderately turbid. Ring and yellow sediment.

Milk: Precipitation of casein; digestion. Alkaline.

Nitrites not produced from nitrates.

Indole not produced.

Hydrogen sulfide produced.

Acid from glucose, galactose, fructose, lactose, maltose, sucrose and glycerol. No acid from mannitol.

Starch hydrolyzed.

Pectate medium not liquefied.

Temperature relations: Optimum, between 25° and 30° C. Maximum, 35° C.

pH range for growth: pH 5.8 to 9.0. Optimum pH, between 6.5 and 7.0.

Slight growth in 5 per cent salt.

Aerobic.

Source: Species first isolated from squash.

Habitat: Causes a leaf spot of squash and related plants.

16. Xanthomonas desmodii Uppal and Patel, 1949. (Uppal and Patel, in Patel, Curr. Sci., 18, 1949, 213; also see Patel, Indian Phytopath., 2, 1949, 5.)

des.mo'di.i. M.L. neut.n. Desmodium generic name of host; M.L. gen.noun desmodii of Desmodium.

Rods, 0.4 to 0.8 by 1.6 to 2.4 microns, occurring singly or in pairs. Motile with a single polar flagellum. Gram-negative.

Gelatin: Liquefied.

Nutrient agar slants: Growth fair, filiform, flat, dull, smooth, opaque and pinard-yellow.

Potato-glucose agar (neutral) colonies: Yellowish amber with colorless margins, circular, viscid, smooth and wet.

Beef broth: Growth slow. Moderate in 48 hours and good in 4 days.

Milk: Litmus turns red in 10 days. Reduction slow.

Indole not produced.

Hydrogen sulfide production fair.

Nitrites not produced from nitrates.

No growth in Cohn's, Uschinsky's or Fermi's solution.

Acid but no gas from glucose, galactose, lactose, mannitol, maltose and sucrose in synthetic medium. Poor growth in salicin, raffinose, fructose, arabinose, xylose, dulcitol and glycerol, and no growth in tartaric,