Page:Bergey's manual of determinative bacteriology.djvu/180

 Source: Isolated from red stripe lesions in sugar cane.

Habitat: Pathogenic on sugar cane.

6. Xanthomonas barbareae Burkholder, 1941. (Phytopath., 31, 1941, 348.)

bar.ba're.ae. M.L. fem.n. Barbarea generic name of cress; M.L. gen.noun barbareae of Barbarea. Rods 0.4 to 0.95 by 1.0 to 3.15 microns. Motile with a single polar flagellum. Gram-negative.

Gelatin: Liquefied.

Beef-extract peptone colonies: Circular, yellow, smooth, butyrous, growth moderate.

Potato glucose agar: Growth abundant, pale yellow. Mucoid.

Broth: Turbid, yellow granular ring.

Milk: Soft curd, with clearing and production of tyrosine crystals. Litmus reduced.

Nitrates utilized but no nitrites produced. Asparagine and nitrites not utilized.

Hydrogen sulfide produced.

Indole not produced.

Lipolytic (Starr and Burkholder, Phytopath., 32, 1942, 600).

Acid from glucose, galactose, xylose, maltose, sucrose and glycerol. Alkali produced from salts of malonic, citric, malic and succinic acids. Rhamnose, salicin and hippuric acid salts not utilized.

Starch hydrolyzed.

Pectate medium liquefied.

Aerobic.

Distinctive characters: Similar to Xanthomonas campestris but does not infect cabbage, cauliflower or horseradish.

Source: From black rot of winter cress, Barbarea vulgaris. Habitat: Pathogenic on leaves and stems of Barbarea vulgaris.

7. Xanthomonas begoniae (Takimoto, 1934) Dowson, 1939. (Bacterium begoniae Takimoto, Jour. Plant Protect., 21, 1934, 262; Dowson, Zent. f. Bakt., II Abt., 100, 1939, 190.)

be.go'ni.ae. Named for Bégon; M.L. fem.n. Begonia generic name; M.L. gen.noun begoniae of Begonia. Translated by Dr. K. Togashi.

Rods 0.5 to 0.6 by 1.2 to 2.0 microns. Motile with a polar flagellum. Gram-negative.

Gelatin: No liquefaction. Liquefaction (Wieringa, Tidschr. Plantziekt., 41, 1935, 312; McCulloch, Jour. Agr. Res., 54, 1937, 859; Dowson, op. cit., 1939, 190; Stapp, Arbeiten Biol. Reichsanst. f. Land- u. Forstw., 22, 1938, 392).

Potato agar colonies: Circular, convex, smooth, moist, shining, yellow.

Broth: Turbid. Yellow pellicle and precipitation.

Milk: No coagulation. Casein digested. Alkaline.

Nitrites not produced from nitrates.

Indole not produced.

Hydrogen sulfide produced.

Lipolytic (Starr and Burkholder, Phytopath., 32, 1942, 600).

No acid or gas in peptone broth from glucose, sucrose, lactose or glycerol. Acid from glucose, sucrose, lactose, mannitol and glycerol in peptone-free medium (McCulloch, op. cit., 1937,859).

Starch hydrolyzed (Dowson, Jour. Roy. Hort. Soc., 63, 1938, 289).

Pectate medium not liquefied.

Temperature relations: Optimum, 27°C. Minimum, between 1° and 3° C. Maximum, 37° C.

Source: Isolated from leaf spot of tuberous begonia.

Habitat: Pathogenic on Begonia spp.

8. Xanthomonas betlicola Patel et al., 1951. (Patel, Kulkarni and Dhande, Curr. Sci., 20, 1951, 106.)

bet.li'co.la. East Indian betle, the name of the betel, a shrubby vine; L. v. colo to dwell; M.L. fem.n. betticola the betel- dweller.

Rods slender, occurring singly or in pairs. Motile. Encapsulated. Gram-negative.

Gelatin: Liquefied.

Potato-glucose agar colonies: 11 mm in diameter in 7 days, baryta-yellow, lobate, striations at periphery.

Broth: Turbid; yellow growth.

Milk: Litmus reduced. Casein digested.

Loeffler's blood serum liquefied.

Indole not produced.

Hydrogen sulfide produced.