Page:Bergey's manual of determinative bacteriology.djvu/172

 Broth: Growth slow with moderate turbidity in 48 hours. A thin pellicle.

Milk: Alkaline and clears.

Nitrites not produced from nitrates.

Indole not produced.

Hydrogen sulfide not produced.

Not lipolytic (Starr and Burkholder, Phytopath., 32, 1942,601).

Acid but no gas from glucose, arabinose, fructose and xylose. No acid from sucrose, maltose, lactose, raffinose, glycerol or mannitol.

Starch partially digested.

Temperature relations: Optimum between 22° and 30° C. Minimum, 1.5° C. Maximum between 37° and 38° C.

Chemical tolerance: Optimum pH between 6.0 and 6.6. Minimum, 5.0. Maximum between 8.3 and 8.6.

Source: Elliott used for her description 4 cultures isolated from lesions on sorgo, sorghum and broom-corn.

Habitat: Pathogenic on sorghum, Holcus sorghum.

143. Pseudomonas lignicola Westerdijk and Buisman, 1929. (De Iepenziekte, Arnhem, 1929, 51.)

lig.ni'co.la. L. lignum wood; L. -cola dweller; M.L. fem.noun lignicola wood dweller or inhabitant.

Rods. Single or short chains. Motile with 1 to several polar flagella. Gram-negative.

Gelatin: No liquefaction.

Malt agar streaks: Milk-white with a colorless edge.

Broth: Turbid with light pellicle.

Milk: No coagulation. No acid.

Nitrites not produced from nitrates.

Indole not produced.

Starch hydrolysis slight.

Optimum temperature, ±25° C.

Source: From vessels of elm wood showing dark discoloration, in Holland.

Habitat: Pathogenic on elm wood.

144. Pseudomonas petasitis (Takimoto, 1927) Săvulescu, 1947. (Bacterium petasitis (sic) Takimoto, Ann. Phyt. Soc. Japan, 2, 1927, 55; Sāvulescu, Anal. Acad. Romane, III, 22, 1947, 11.)

pe.ta'si.tis. Gr. mas.noun petasus a sombrero, a broad-brimmed felt hat; Gr. mas.noun petasites a broad-leafed plant, colt's foot; M.L. mas.noun Petasites generic name; M.L. gen.noun petasitis of Petasites. Rods 0.8 to 1.1 by 1.1 to 1.7 microns. Motile with a polar flagellum. Gram-negative. Gelatin: No liquefaction.

Beef agar colonies: White, circular or amoeboid, butyrous.

Broth: Strong turbidity. Pellicle.

Milk: Coagulated in 30 days.

Nitrites produced from nitrates with gas formation.

Indole not produced.

Hydrogen sulfide not produced.

No evident acid in peptone broth, but gas from glucose, lactose and sucrose. Acid but no gas from glycerol.

Weak growth in broth plus 6 per cent salt.

Temperature relations: Optimum between 27° and 30° C. Minimum, approximately 5° C. Maximum, 47° C.

Source: Isolated from brown to black lesions on Petasites japonicus in Japan.

Habitat: Pathogenic on leaves of Petasites japonicus.

145. Pseudomonas woodsii (Erw. Smith, 1911) Stevens, 1925. (Bacterium woodsii Erw. Smith, Bacteria in Relation to Plant Diseases, 2, 1911, 62; Stevens, Plant Disease Fungi, New York, 1925, 39.)

wood'si.i. Named for A. F. Woods, an American plant pathologist; M.L. gen.noun woodsii of Woods.

Description from Burkholder and Guterman (Phytopath., 25, 1935, 118).

Rods 0.67 by 1.56 microns. Motile with a polar flagellum. Gram-negative.

Gelatin: No liquefaction.

Beef-extract agar slants: Growth slow and scant, filiform, creamy, butyrous.

Broth: Turbid.

Milk: Becomes alkaline but otherwise little changed.

Nitrites not produced from nitrates.

Indole not produced.

Hydrogen sulfide not produced.

Not lipolytic (Starr and Burkholder, Phytopath., 32, 1942, 601).

Acid but no gas from glucose, fructose, galactose, arabinose, xylose, rhamnose, lactose, glycerol and mannitol. Alkaline