Page:Bergey's manual of determinative bacteriology.djvu/171

 Indole not produced.

Hydrogen sulfide not produced.

Acid but no gas from sucrose and glycerol. No acid from lactose and maltose.

Starch hydrolyzed.

Temperature relations: Optimum between 27° and 28° C. Minimum, 12° C. Maximum, 35.5° C.

Chemical tolerance: Good growth at pH 6.4. No growth at pH 5.4 and pH 8.8

Habitat: Pathogenic on sunflower, Helianthus debilis.

140. Pseudomonas melophthora Allen and Riker, 1932. (Phytopath., 22, 1932, 557.)

me.loph'tho.ra. Gr. melum apple; Gr. phthora decay, destruction; M.L. adj. melophthonis apple-destroying.

Rods 0.68 by 1.32 microns. Motile with 2 polar flagella. Gram-negative; Gram-positive cells appear in old cultures.

Gelatin: No liquefaction.

Nutrient agar plus 2 per cent glucose: Colonies appear in 36 hours. After 3 days colonies circular, smooth, glistening, convex; edges entire; light pink, but not constant.

Broth: Good growth. Pellicle and sediment.

Milk: Little change, if any.

Nitrites not produced from nitrates.

Indole not produced.

Hydrogen sulfide not produced.

Acid from arabinose, glucose, galactose, fructose, sucrose and glycerol. No acid from lactose, maltose, dextrin or inulin.

Starch not hydrolyzed.

Optimum temperature between 21° and 25° C.

Source: Description based on 7 cultures isolated from rotting apples and from apple maggots.

Habitat: Pathogenic on apples and found with the apple maggot, Rhagoletis pomonella.

141. Pseudomonas alboprecipitans Rosen, 1922. (Ann. Missouri Bot. Garden, 9, 1922, 383.)

al.bo.pre.ci'pi.tans. L. album the color white; L. v. praecipito to precipitate; M.L. part.adj. alboprecipitans forming a white sediment.

Description revised in accordance with Johnson, Roberts and Cash (Jour. Agr. Res., 78, 1949, 723).

Rods, 0.6 by 1.8 microns, occurring singly or in pairs. Encapsulated. Motile with a polar flagellum. Gram-negative.

Gelatin: Liquefied

Nutrient agar colonies: White, circular, raised, smooth, sticky, with margins entire. Whitish discoloration of the medium.

Broth: Turbid in 24 hours. Heavy sediment in old cultures.

Uschinsky's solution: Turbid in 24 hours; pellicle formed.

Cohn's and Fermi's solutions: No growth.

Milk: Becomes alkaline and slowly clears.

Nitrites produced from nitrates.

Indole not produced.

Hydrogen sulfide production slight.

Acid but no gas from glucose, galactose, fructose, sucrose, lactose, raffinose, glycerol and mannitol. No acid from maltose.

Starch hydrolyzed.

Temperature relations: Optimum between 30° and 35° C. Minimum, 0° C. Maximum, 40° C. Aerobic.

Distinctive character: White precipitate in culture media.

Source: Isolated a number of times from foxtail grass.

Habitat: Pathogenic on foxtail, Chactochloa lutescens, and other grasses.

142. Pseudomonas andropogonis (Erw. Smith, 1911) Stapp, 1928. (Bacterium andropogoni (sic) Erw. Smith, Bacteria in Relation to Plant Diseases, 2, 1911, 63; Elliott and Smith, Jour. Agr. Res., 38, 1929, 4; Pseudomonas andropogoni (sic) Stapp, in Sorauer, Handbuch der Pflanzenkrankheiten, 2, 5 Aufl., 1928, 27.) an.dro.po.go'nis. Gr. anēr, andris a man; Gr. mas.noun pōgōn, pōgōnis beard; M.L. mas.noun Andrōpōgon, -ōnis man's beard, generic name; M.L. gen.noun andropogonis of Andropogon. Description from Elliott and Smith (op. cit., 1929, 4).

Rods 0.64 by 1.76 microns. Motile with one to several bipolar flagella. Encapsulated. Gram-negative.

Gelatin: Feeble liquefaction or none.

Beef-extract agar colonies: Slow growing, round, smooth, glistening, viscid, white.