Page:Bergey's manual of determinative bacteriology.djvu/167

 Motile with 1 to 5 polar flagella. Encapsulated. Gram-negative.

Gelatin: Liquefied.

Beef-peptone agar colonies: Small, flat, smooth, dry, shining, translucent, grayish and butyrous.

Broth: Turbid in 4 days. Transient pellicle.

Milk: Slightly alkaline. No coagulation nor clearing.

Nitrites not produced from nitrates. No growth on synthetic nitrate agar.

Indole not produced.

Hydrogen sulfide not produced.

Acid reaction occurs in galactose, starch and sucrose. No gas.

Starch not hydrolyzed.

Source: From diseased leaves and fruit of the passion fruit in New Zealand.

Habitat: Pathogenic on Passiflora edulis.

127. Pseudomonas seminum Cayley, 1917. (Jour. Agr. Sci., 8, 1917, 461.)

se'mi.num. L. semen, seminis seed; L. gen.pl. seminum of seeds.

Rods 1.0 by 4.0 to 5.0 microns. Spore-like bodies present. Encapsulated. Motile with a single flagellum. Reported as Gram-positive; however, probably in error. No cultures are available for a retest of this character.

Gelatin: Rapid liquefaction.

Agar colonies: White, more or less circular, transparent, spreading.

Broth: Turbid. Pellicle.

Litmus milk: Milk becomes clear and apricot color.

Nitrites produced from nitrates.

Acid but no gas from glucose and sucrose.

No acid from lactose.

Starch: No hydrolysis.

Optimum temperature, 25° C.

Aerobic, facultative.

Source: Isolated from seeds, stems and pods of diseased peas in England.

128. Pseudomonas vitiswoodrowii Pateld and Kulkarni, 1951. (Pseudomonas vitiswoodrowii (sic) Patel and Kulkaiui, Curr. Sci., 20, 1951, 132.)

vi.tis.wood.ro'wi.i. L. fem.noun vitis a vine; M.L. fem.noun Vitis a generic name; Woodrow patronymic; M.L. gen.noun woodrowii of Woodrow.

Rods 0.8 by 1.5 microns. Motile with a single polar flagellum. Gram-negative.

Gelatin: Liquefied.

Potato dextrose agar colonies: Circular, capitate with margins entire. Pale, dull gray. 1.2 cm in diameter in 7 days.

Broth: Turbid.

Potato cylinders: Scant growth. Medium dark gray.

Milk : Litmus reduced and casein digested.

Hydrogen sulfide produced.

Loeffler's blood serum: Liquefied.

Indole not produced.

Synthetic asparagine medium. No growth.

Methyl red test negative; acetylmethylcarbinol not produced.

Nitrites not produced from nitrates.

Acid but no gas from glucose, lactose and sucrose, but no growth in salicin.

Salt tolerance: Up to 1 per cent.

Optimum temperature between 25° and 28° C.

Aerobic.

Source: Isolated from leaves of Vitis woodrowii in India.

Habitat: Pathogenic on V. woodrowii but not on V. vinifera.

129. Pseudomonas fabae (Yu, 1936) Burkholder, 1948. (Phytomonas fabae Yu, Bull, of the Chinese Bot. Soc, 2, 1936, 34; Burkholder, in Manual, 6th ed., 1948, 139.)

fa'bae. L. faba the horse bean; M.L. gen.noun fabae of the horse bean.

Rods 0.8 to 1.1 by 1.1 to 2.8 microns. Motile with 1 to 4 polar flagella. Gram-negative.

Gelatin: Liquefied.

Nutrient agar colonies: Circular, entire, viscid, glistening, raised, smooth to wrinkled, white to salmon. Medium amber. Broth: Turbid after 12 hours. Pellicle.

Milk: Growth slow. Clears.

Nitrites produced from nitrates.

Indole production slight.

Hydrogen sulfide not produced.

Acid but no gas from glucose. No acid nor gas developed from arabinose, xylose, fructose, galactose, sucrose, lactose, maltose, raffinose, dextrin, inulin, mannitol or