Page:Bergey's manual of determinative bacteriology.djvu/165

 (Phytomonas alliicola Burkholder, Phytopath., 32, 1942, 146; Starr and Burkholder, Phytopath., ibid., 601.)

al.li.i'co.la. L. allium onion; L. -cola dweller; M.L. fem.noun alliicola onion dweller.

Rods 0.7 to 1.4 by 1.05 to 2.8 microns. Motile with 1 to several polar flagella, at times bipolar. Gram-negative.

Gelatin: Liquefied.

Beef-extract peptone agar streaks: Moderate in growth, white at first, later dirty in appearance, edges wavy, consistency viscid. Medium deep brown.

Potato-glucose agar frequently becomes greenish.

Broth: Turbid with light pellicle. Brown.

Milk: Cleared and litmus reduced. Neutral.

Nitrites produced from nitrates.

Indole not produced.

Hydrogen sulfide not produced.

Lipolytic action very strong.

Acid but no gas from l-arabinose, d-xylose, rhamnose, glucose, d-galactose, fructose, d-lactose, maltose, sucrose, glycerol, mannitol and salicin. Alkali from salts of acetic, citric, formic, hippuric, lactic, malic, succinic and tartaric acids.

Starch not hydrolyzed.

Slight growth in broth plus 4 per cent salt.

Aerobic.

Temperature relations: Optimum, 30° C. Minimum, 5° C. Maximum, 41° C.

Source: Seven isolates from storage rot of onion bulbs.

Habitat: Pathogenic on onion bulbs, Allium cepa.

122. Pseudomonas gardeniae Burkholder and Pirone, 1941. (Phytopath., 31, 1941, 194.)

gar.de'ni.ae. L. Garden patronymic; M.L. fem.noun Gardenia plant generic name; M.L. gen.noun gardeniae of Gardenia.

Rods 0.75 by 2.4 microns. Motile with 1 to 2 polar flagella. Gram-negative.

Gelatin: Liquefied.

Beef-extract peptone agar colonies: Growth fair, white to dirty gray and viscid. Medium becoming dark brown.

Potato-glucose agar: No brown color.

Broth: Turbid with pellicle. Dark brown.

Milk: Soft curd with pellicle. Clears in zones. Litmus reduced.

Nitrites produced from nitrates.

Hydrogen sulfide not produced.

Indole not produced.

Acid from glucose, galactose, xylose, rhamnose, sucrose, maltose, mannitol, glycerol and salicin. Alkali produced from the salts of citric, malic, malonic, succinic, tartaric and hippuric acids. Good growth in tyrosine and in asparagine broth.

Starch not hydrolyzed.

Aerobic.

Source: Eight isolates from leaf spots of gardenias in New Jersey.

Habitat: Pathogenic on leaves of Gardenia jasminoides.

123. Pseudomonas caryophylli (Burkholder, 1942) Starr and Burkholder, 1942. (Phytomonas caryophylli Burkholder, Phytopath., 32, 1942, 143; Starr and Burkholder, ibid., 601.)

ca.ry.o'phyl.li. Gr. caryophyllum nut leaf, the clover tree; M.L. mas.noun caryophyllus specific epithet in Dianthus caryophyllus, the clove-pink or carnation; M.L. gen.noun caryophylli of the carnation.

Rods 0.35 to 0.95 by 1.05 to 3.18 microns. At times slightly curved. Motile with 1 to several polar flagella. Frequently bipolar. Gram-negative.

Gelatin: Liquefaction after 3 to 4 weeks.

Potato glucose agar colonies: 3 to 4 mm in diameter, circular, smooth, glistening, edges entire. Color is tan to gray-mauve. Old culture dark brown. Consistency butyrous.

Broth: Turbid with a white sediment.

Milk: Litmus slowly becomes blue. Slight reduction at bottom of tube. No clearing.

Nitrites produced from nitrates. Also ammonia and gas are produced in a synthetic nitrate medium. Asparagine, KNO3 and NH4H2PO4 can be utilized.

Indole not produced.

Hydrogen sulfide not produced.

Lipolytic action slight to moderate.

Acid from l-arabinose, d-xylose, rhamnose, glucose, d-galactose, fructose, d-lactose, maltose, sucrose, glycerol, mannitol and salicin. Alkali with sodium salts of