Page:Bergey's manual of determinative bacteriology.djvu/163

 Rods 0.75 to 1.5 by 1.5 to 3.75 microns. Motile with 1 or 2 polar fiagella. Gram-negative.

Green fluorescent pigment produced in culture.

Gelatin: No liquefaction.

Beef-extract agar colonies: Circular, grayish white with bluish tinge, raised with slightly irregular edges.

Broth: Turbid in 36 hours with a smooth, viscous pellicle.

Milk: Alkaline.

Nitrites not produced from nitrates.

Indole not produced.

Hydrogen sulfide not produced.

Not lipolytic (Starr and Burkholder, Phytopath., 32, 1942, 601).

Acid but no gas from glucose, galactose fructose, mannose, arabinose, xylose, mannitol and glycerol. Alkaline production from salts of acetic, citric, lactic, malic, succinic and tartaric acids. Rhamnose, maltose, sucrose, lactose, raffinose and salicin not utilized. Starch not hydrolyzed.

Slight growth in broth plus 6 per cent NaCl.

Chemical tolerance: Optimum pH, between 6.8 and 7.1. Minimum, between 5.0 and 5.3. Maximum, between 9.2 and 9.4. (Kotte, op. cit., 2, 1930, 453).

Aerobic, facultative.

Source: Isolated from rot of French endive, Cichorium intybus, by Swingle and by Okabe, and from C. endivia by Kotte.

Habitat: Pathogenic on endive, lettuce and larkspur.

116. Pseudomonas nectarophila (Doidge, 1917) Rosen and Bleeker, 1933. (Bacterium nectarophilum Doidge, Ann. Appl. Biol., 4, 1917, 73; Rosen and Bleeker, Jour. Agr. Res., 46, 1933, 98.)

nec.ta.ro'phi.la. Gr. nectar nectar; Gr. adj. philus loving; M.L. adj. nectarophilus nectar-loving.

Rods 0.5 to 0.7 by 0.6 to 1.5 microns.

Motile with 1 to 5 polar flagella. Encapsulated. Gram-negative.

Green fluorescent pigment produced in culture.

Gelatin: No liquefaction.

Nutrient agar colonies: Yellowish white, wet-shining, smooth; margins irregular.

Broth: Heavy turbidity in 24 hours. Sediment.

Milk: Cleared.

Nitrites not produced from nitrates.

Indole not produced.

Acid from glucose and galactose. No acid from sucrose.

Starch hydrolysis feeble.

Optimum temperature between 25° and 30° C.

Aerobic, facultative.

Distinctive character: Differs from Pseudomonas barkeri in that it does not liquefy gelatin nor produce indole. Produces capsules.

Source: Isolated from blighted pear blossoms in South Africa.

Habitat: Pathogenic on pear blossoms.

117. Pseudomonas viburni (Thornberry and Anderson, 1931) Stapp, 1935. (Phytomonas viburni Thornberry and Anderson, Phytopath., 21, 1931, 912; Stapp, Bot. Rev. 1, 1935, 407.)

vi.bur'ni. L. viburnum way-faring tree; M.L. neut.noun Viburnum name of a genus of flowering plants; M.L. gen.noun viburni of Viburnum.

Rods 0.5 to 1.0 by 1.0 to 2.0 microns. Encapsulated. Motile with 2 to 4 polar flagella. Gram-negative (Burkholder); not Gram-positive as stated in original.

Green fluorescent pigment produced in culture (Burkholder).

Gelatin: No liquefaction.

Glucose beef-extract colonies: Dull gray, circular, edges entire.

Broth: Turbid with pellicle.

Milk: Alkaline.

Nitrites not produced from nitrates.

Indole not produced.

Hydrogen sulfide not produced.

Not lipolytic (Starr and Burkholder, Phytopath., 32, 1942, 601).

Acid from glucose and galactose but not sucrose (Burkholder).

Starch: No hydrolysis.

Slight growth in 3.5 per cent salt (Burkholder).

Temperature relations: Optimum, 25° C. Minimum, 12° C. Maximum, 35° C.