Page:Bergey's manual of determinative bacteriology.djvu/161

 Starch hydrolysis feeble.

Temperature relations : Optimum between 27° and 28° C. Maximum, 37° C.

Chemical tolerance: Optimum pH between 6.2 and 6.8. pH range, 5.4 to 8.9.

Source: Isolations from naturally infected tung oil trees in Georgia.

Habitat: Pathogenic on the tung oil tree (Aleurites fordi), on the bean (Phaseolus vulgaris) and the castor bean (Ricinus communis).

110. Pseudomonas glycinea Coerper, 1919. (Jour. Agr. Res., 18, 1919, 188.)

gly.ci'ne.a. Gr. glycys sweet; M.L. fem.noun Glycine generic name of a legume; M.L. adj. glycinea of the soybean.

Rods 1.2 to 1.5 by 2.3 to 3.0 microns. Motile with polar flagella. Gram-negative.

Green fluorescent pigment produced in culture.

Gelatin: Not liquefied.

Beef-peptone agar colonies: Appear in 24 hours. Circular, creamy white, smooth, shining and convex. Margins entire. Butyrous in consistency.

Milk: Litmus turns blue and later a separation of the milk occurs. Casein not digested.

Nitrites produced from nitrates (Burkholder and Starr, Phytopath.,38, 1948, 498).

Indole test feebly positive.

Not lipolytic (Starr and Burkholder, Phytopath., 32, 1942, 601).

Acid from glucose and sucrose.

Starch not hydrolyzed.

Temperature relations: Optimum between 24° and 26° C. Minimum, 2° C. Maximum, 35° C.

Aerobic, facultative.

Comment: A variety of this species that differs slightly in morphology, action in milk and in chromogenesis has been described by Takimoto (Jour. Plant Prot., Tokyo, 14, 1927 556). It was isolated from leaf spots on soy bean in Formosa.

Source: A number of cultures isolated from soybeans in Wisconsin.

Habitat: Pathogenic on soybean, Glycine max (Soja max).

111. Pseudomonas savastanoi (Erw. Smith, 1908) Stevens, 1913. (Bacterium savastanoi Erw. Smith, U. S. Dept. Agr. Plant Ind. Bull. 131, 1908, 31; Stevens, The Fungi which Cause Plant Diseases, 1913, 33.)

sa.vas.ta'no.i. Savastano patronymic; savastanoi of Savastano.

Description from Brown (Jour. Agr. Res., '44', 1932, 711).

Rods 0.4 to 0.8 by 1.2 to 3.3 microns. Motile with 1 to 4 polar flagella. Gram-negative.

Green fluorescent pigment found in culture.

Gelatin: No liquefaction.

Beef agar colonies: White, smooth, flat, glistening, margins erose or entire. Broth: Turbid on the second day. No pellicle or ring.

Milk: Becomes alkaline.

Nitrites not produced from nitrates.

Hydrogen sulfide not produced.

Acid but no gas from glucose, galactose and sucrose.

Starch hydrolyzed.

Temperature relations: Optimum between 23° and 24° C. Minimum, 1° C. Maximum, 32° C.

Chemical tolerance: Optimum between 6.8 and 7.0. Minimum, 5.6. Maximum, 8.5.

Aerobic.

Comment: A variety that differs but slightly from this species is described as pathogenic on ash, Fraxinus excelsior and F. americana, but not on olive. Produces a canker on ash. See Brown (Jour. Agr. Res., 4, 1932, 721).

Source: Smith isolated his cultures from olive galls collected in California.

Habitat: Pathogenic on olive.

112. Pseudomonas tonelliana (Ferraris, 1926) Burkholder, 1948. (Bacterium tonellianum Ferraris, Trattato di Patologia e Terapia Vegetale, 3rd ed., 1, 1926, 104; Burkholder, in Manual, 6th ed., 1948, 132.)

to.nel.li.a'na. M.L. adj. tonellianus pertaining to Tonelli; named for A. Tonelli.

Description from C. O. Smith (Phytopath., 18, 1928, 503) unless otherwise noted.

Rods 0.5 to 0.6 by 1.5 to 2.5 microns. Motile with 1 to 3 polar flagella. Gram-negative (Adam and Pugsley, Jour. Dept. Agr. Victoria, 32, 1934, 304).