Page:Bergey's manual of determinative bacteriology.djvu/159

 Starch slowly digested.

Source: Barker made many cultures from blighted pear blossoms. Doidge received a culture from Barker.

Habitat: Causes a blossom blight of pear.

103. Pseudomonas betle (Ragunathan, 1928) Burkholder, 1948. (Bacterium betle Ragunathan, Ann. Roy. Gard., Peradeniya, Ceylon, 11, 1928, 51; Burkholder, in Manual, 6th ed., 1948, 130.)

bet'le. Malayan noun betle betel; M.L. indeclin.noun betle.

Rods, 0.5 by 1.5 to 2.5 microns, occurring singly or in short chains. Non-motile. Gram-negative.

Green pigment formed in nutrient gelatin and in broth.

Gelatin: Liquefied.

Bovril agar colonies: Honey-yellow, circular at first, later echinulate. Raised, smooth and shiny.

Broth: Surface becomes cloudy in 2 days. Pellicle.

No gas from lactose, maltose or sucrose.

Starch is reduced.

Aerobic.

Source: Five cultures isolated from leaf spots on the betel vine.

Habitat: Pathogenic on the betel vine, Piper betle.

104. Pseudomonas gladioli Severini, 1913. (Annali d. Bot., Rome, 11, 1913, 420.)

gla.di'o.li. L. gladiolus a small sword lily; M.L. mas.n. Gladiolus generic name of gladiolus; M.L. gen.noun gladioli of gladiolus.

Rods 0.6 by 2.3 to 2.8 microns. Motile with one or more polar flagella. Gram-negative.

A pale yellow, water-soluble pigment found, later orange.

Gelatin colonies: Cream-colored, wart-like. Rapid liquefaction.

Milk: Coagulated and slowly peptonized.

Nitrites not produced from nitrates.

Indole not produced.

No gas.

Aerobic.

Optimum temperature between 28° and 30° C.

Habitat: Causes a corm rot of gladiolus and other tubers.

105. Pseudomonas mellea Johnson, 1923. (Jour. Agr. Res., 23, 1923, 489.)

mel'le.a. L. adj. melleus pertaining to honey.

Rods 0.6 by 1.8 microns. Encapsulated. Motile with 1 to 7 polar flagella. Gram-negative.

Green fluorescent pigment produced in culture.

Gelatin: Liquefied.

Potato-glucose agar: Growth abundant, smooth, glistening, viscid, honey-colored.

Broth: Turbid in 24 hours. Pellicle.

Milk: Alkaline; clears.

Nitrites not produced from nitrates.

Indole not produced.

Hydrogen sulfide not produced.

Starch hydrolysis feeble.

Growth inhibited by 4 per cent salt.

Temperature relations: Optimum between 26° and 28° C. Maximum, 36° C.

Aerobic, facultative.

Distinctive character: Differs from Pseudomonas pseudozoogloeae in that it produces on tobacco a brown instead of a black spot with a halo, is orange-yellow in culture, and turns milk alkaline.

Source: Isolated from brown rusty spots on tobacco in Wisconsin.

Habitat: Pathogenic on leaves of tobacco, Nicotiana tobacum.

106. Pseudomonas panacis (Nakata and Takimoto, 1922) Dowson, 1943. (Bacterium panaxi Nakata and Takimoto, Bull. Agr. Sta. Chosen, 5, 1922, 1; Dowson, Trans. Brit. Mycol. Soc, 26, 1943, 10.)

pa'na.cis. Gr. panax the plant heal-all; M.L. neut.noun Panax a generic name; M.L. gen.noun panacis of Panax.

Description from Elliott (Bact. Plant Pathogens, 1930, 173).

Rods 0.5 by 1.3 to 1.5 microns. Chains. Motile with 4 to 6 polar flagella. Gram-negative.

Green fluorescent pigment produced in culture.

Gelatin: Slight liquefaction.

Agar colonies: White.

Milk: Coagulated.

No gas from sugars.