Page:Bergey's manual of determinative bacteriology.djvu/157

 Beef-extract agar colonies: Growth moderate. Milky white, circular, convex.

Broth: Turbid in 24 hours. No pellicle.

Milk: Coagulated in 6 days and later digested. Litmus slightly acid.

Nitrites not produced from nitrates.

Indole not produced.

Ammonia not produced.

No gas from carbohydrates. Acid from glucose, galactose, arabinose and mannose. No acid from sucrose, maltose, lactose, raffinose, mannitol or salicin.

Source: From diseased Japanese privet in Lisbon, Portugal.

Habitat: Pathogenic on privet, Ligustrum japonicum.

97. Pseudomonas marginalis (Brown, 1918) Stevens, 1925. (Bacterium marginale Brown, Jour. Agr. Res., 13, 1918, 386; Stevens, Plant Disease Fungi, New York, 1925, 30; Phytomonas intybi Swingle, Phytopath., 15, 1925, 730.)

mar.gi.na'lis. L. margo, marginis edge, margin; M.L. adj. marginalis marginal.

Description from Brown (op. cit., 1918, 386) and Clara (Cornell Agr. Exp. Sta. Mem. 159, 1934, 27).

Rods. Motile with 1 to 3 polar flagella. Gram-negative.

Green fluorescent pigment produced in culture.

Gelatin: Liquefied.

Agar colonies: Cream-colored to yellowish.

Broth: Turbid, with pellicle.

Milk: Alkaline. Soft curd at times.

Nitrites are produced from nitrates. Not produced (Clara).

Indole not produced.

Hydrogen sulfide not produced.

Acid but no gas from glucose, galactose, fructose, mannose, arabinose, xylose, rhamnose, mannitol and glycerol. Alkali from salts of acetic, citric, malic, formic, lactic, succinic and tartaric acids. Sucrose, maltose, lactose, raffinose and salicin not fermented (Clara).

Starch hydrolysis feeble. None (Clara).

Temperature relations: Optimum between 25° and 26° C. Minimum, 0° C. Maximum, 38° C.

Aerobic.

Source: Isolated from marginal lesion on lettuce from Kansas.

Habitat: Pathogenic on lettuce and related plants.

98. Pseudomonas sesami Malkoff, 1906. (Cent. f. Bakt., II Abt., 16, 1906, 665.)

se'sa.mi. Gr. sesamum sesame; M.L. neut.noun Sesamum generic name of sesame; sesami of sesame.

Description from Nakata (Ann. Phyt. Soc. Japan, 2, 1930, 242).

Rods 0.6 to 0.8 by 1.2 to 3.8 microns. Motile with 2 to 5 polar flagella. Gram-negative.

Green fluorescent pigment produced in culture.

Gelatin: Liquefaction rapid.

Beef-agar colonies: Circular, flat, striate, smooth, entire margins, white.

Broth: Growth rapid. No pellicle.

Milk: Alkaline. No coagulation.

Nitrites not produced from nitrates.

Indole not produced.

Hydrogen sulfide not produced.

Acid but no gas from glucose. No acid from lactose, sucrose or glycerol. Starch not hydrolyzed.

Temperature relations: Optimum, 30° C. Minimum, 0° C. Maximum, 35° C.

Aerobic, facultative.

Source: Isolated from brown spots on leaves and stems of sesame.

Habitat: Pathogenic on sesame.

99. Pseudomonas setariae (Okabe, 1934) Săvulescu, 1947. (Bacterium setariae Okabe, Jour. Soc. Trop. Agr. Formosa, 6, 1934, 63; Săvulescu, Anal. Acad. Romane, III, 22, 1947, 11.)

se.ta'ri.ae. L. saeta a bristle; M.L. saetarius bristle-like; M.L. fem.noun Setaria generic name of foxtail; setariae of Setaria. Rods 0.4 to 0.8 by 1.8 to 4.4 microns. Motile with a polar, seldom bipolar, flagellum. Gram-negative.

Yellowish, water-soluble pigment produced in culture.

Gelatin: Slow liquefaction.

Beef-extract agar colonies: Circular, white, opalescent, smooth, glistening.

Broth: Turbid after 18 hours. Pellicle.