Page:Bergey's manual of determinative bacteriology.djvu/156

 acids. Sucrose, lactose, maltose, raffinose, salicin, and salts of formic and tartaric acids not fermented.

Starch: No hydrolysis.

Growth in broth plus 5 per cent NaCl.

Aerobic, facultative.

Comment: A variety that does not grow in Uschinsky's solution and that produces colonies of an unusual shape has been isolated from the stems and leaves of blighted beans in Denmark. See Petersen (Tidsskr. f. Planteavl., 38, 1932, 851).

Source: Two cultures isolated from spotted beans, one from England and one from Switzerland.

Habitat: Pathogenic on bean, Phaseolus vulgaris.

94. Pseudomonas ananas Serrano, 1934. (Philipp. Jour. Sci., 55, 1934, 355.)

a'na.nas. Braz.Ind. ananas pineapple; M.L. indecl.neut.noun ananas. Rods 0.6 by 1.8 microns. Motile with 1 to 4 polar flagella. Gram-negative.

Green fluorescent pigment produced in certain media.

Gelatin: Liquefied.

Beef-extract glucose agar colonies: White, with undulating edges, smooth to rugose, glistening to dull.

Beef-extract agar: Growth scant.

Broth: Feeble growth.

Milk: Becomes alkaline with curd.

Nitrites not produced from nitrates.

Indole not produced.

Hydrogen sulfide not produced.

Acid but no gas from glucose, xylose and mannitol. Feeble with lactose. No acid from sucrose.

Starch not hydrolyzed.

Temperature relations: Optimum between 30° and 31° C. Minimum between 7° and 10° C. Maximum, 45° C.

Aerobic.

Source: Isolated from rotted pineapples.

Habitat: Causes a rot of pineapples, Ananas comosus.

95. Pseudomonas bowlesiae (Lewis and Watson, 1927) Dowson, 1943. (Phytomonas bowlesii (sic) Lewis and Watson, Phytopath., 17, 1927, 511; Pseudomonas bowlesiae Dowson, Trans. Brit. Mycol. Soc, 26, 1943, 9.)

bow.le'si.ae. M.L. fem.n. Bowlesia generic name; M.L. gen.noun bowlesiae of Bowlesia.

Rods, 0.5 to 0.7 by 1.2 to 1.6 microns, occurring singly, in pairs or in short chains. Motile with bipolar flagella. Gram-negative.

Green fluorescent pigment produced in culture.

Gelatin: Liquefied.

Agar slants: Yellowish, moist, glistening and viscid.

Broth: Uniform turbidity throughout. Heavy viscous sediment in old cultures.

Milk: Alkaline; coagulation with a slow peptonization.

Nitrites produced from nitrates.

Indole produced.

Hydrogen sulfide produced.

Acid from glucose, maltose and xylose. No acid from sucrose.

Temperature relations: Optimum, 27° C. Minimum, —1° C. Maximum, 37° C.

Chemical tolerance: Optimum pH, 7.2. pH range, 4.5 to 8.6.

Aerobic.

Source: Isolated from diseased, water-soaked spots of bowlesia.

Habitat: Pathogenic on Bowlesia septentrionalis.

96. Pseudomonas ligustri (d'Oliveira, 1936) Săvulescu, 1947. (Bacterium ligustri d'Oliveira, Revista Agron., 24, 1936, 434; Săvulescu, Anal. Acad. Romane, III, 22, 1947, 11.)

li.gus'tri. L. ligustrum the privet; M.L. neut.noun Ligustrum generic name of privet; ligustri of the privet.

Rods 0.5 to 0.7 by 1.3 to 3.0 microns. No chains. Not encapsulated. Motile with 2 to 5 polar flagella. Gram-negative.

Green pigment produced on Dox agar and in broth.

Gelatin: Liquefied.