Page:Bergey's manual of determinative bacteriology.djvu/155

 Rods 1.2 by 3.3 microns. Motile with a polar flagellum. Gram-negative.

Gelatin: Liquefied.

Potato agar colonies: Grayish white, circular, raised, wet-shining, smooth. Milk: Alkaline; clears.

Nitrites produced from nitrates (Burkholder and Starr, Phytopath., 38, 1948, 498).

Indole not produced.

Acid from glucose, galactose, fructose, l-arabinose, xylose, sucrose, pectin, mannitol and glycerol (Braun, Phytopath., 27, 1937, 289).

Ammonium sulfate, potassium nitrate, cystine, glutamic acid, glycine, succinimide, oxamide, acetamide and urea can be used as nitrogen sources (Braun).

Starch not hydrolyzed.

Aerobic.

Relationship to other species: Braun (loc. cit.) states that Pseudomonas tabaci and Pseudomonas angulata are identical in culture.

Source: Isolated from wildfire lesions and tobacco leaves in North Carolina.

Habitat: Pathogenic on tobacco, Nicotiana tabacum.

92. Pseudomonas polycolor Clara, 1930. (Phytopath., 20, 1930, 704.)

po.ly'co.lor. Gr. poly- many; L. color color; M.L. adj. polycolor many colored.

Description taken from Clara (Cornell Agr. Exp. Sta. Mem. 159, 1934, 28).

Rods 0.75 to 1.2 by 1.05 to 3.0 microns. Motile with 1 or 2 polar flagella. Gram-negative.

Green fluorescent pigment produced in culture.

Gelatin: Liquefied.

Beef-extract agar colonies: Grayish white, circular, raised; thin, transparent margins.

Broth: Turbid in 36 hours with thin pellicle.

Milk: Alkaline; no curd.

Nitrites not produced from nitrates.

Indole not produced.

Hydrogen sulfide not produced.

Lipolytic (Starr and Burkholder, Phytopath., 32, 1942, 601).

Acid but no gas from glucose, galactose. fructose, mannose, arabinose, xylose, mannitol and glycerol. Alkaline reaction from salts of acetic, citric, malic, lactic and formic acids. Rhamnose, sucrose, maltose, lactose, raffinose and salicin not fermented.

Starch not hydrolyzed.

Aerobic, facultative.

Good growth in broth plus 7 per cent salt.

Temperature relations: Optimum between 25° and 30° C. Maximum between 37° and 39° C.

Distinctive characters: Differs from Pseudomonas mellea in type of lesion produced; does not digest starch nor reduce nitrates and does not form acid from lactose nor sucrose. Pathogenic for laboratory animals (Elrod and Braun, Sci., 94, 1941, 520). Cultural characters differ from those of Pseudomonas aeruginosa Migula.

Source: Repeatedly isolated from leaf spot of tobacco in the Philippines.

Habitat: Pathogenic on tobacco.

93. Pseudomonas viridiflava (Burkholder, 1930) Clara, 1934. (Phytomonas viridiflava Burkholder, Cornell Agr. Exp. Sta. Mem. 127, 1930, 63; Clara, Science, 75, 1934, 111.)

vi.ri.di.fla'va. L. viridis green; L. flavus yellow; M.L. adj. viridiflavus greenish yellow.

Description from Clara (Cornell Agr. Exp. Sta. Mem. 139, 1934, 30).

Rods 0.75 to 1.5 by 1.5 to 3.15 microns. Motile with 1 or 2 polar flagella. Gram-negative.

Green fluorescent pigment produced in culture.

Gelatin: Liquefied.

Beef-extract agar colonies: Grayish white, margins corrugated, edges irregular.

Broth: Turbid in 36 hours.

Milk: Becomes alkaline and clears.

Nitrites not produced from nitrates.

Indole not produced.

Hydrogen sulfide not produced.

Not lipolytic (Starr and Burkholder, Phytopath., 32, 1942, 601).

Acid but no gas from glucose, fructose, mannose, arabinose, xylose, mannitol and glycerol. Alkaline reaction from salts of acetic, citric, malic, lactic and succinic