Page:Bergey's manual of determinative bacteriology.djvu/153

, sucrose, raffinose, starch, salicin or tartaric acid (Mushin).

Temperature relations : Optimum, between 20° and 24° C. Minimum, below 0° C. Maximum, 38.5° C. (Mushin).

Limits of growth in broth are pH 4.4 to pH 9.5 (Mushin).

Aerobic.

Source: Isolated from vascular and parenchymatic disease of stocks, Matthiola incana var. annua. Habitat: Pathogenic on stocks.

86. Pseudomonas morsprunorum Wormald, 1931. (Pseudomonas mors-prunorum (sic) Wormald, Jour. Pom. and Hort. Sci., 9, 1931, 251.)

mors'pru.no.rum. L. mors death; L. prunus plum; M.L. fem.noun morsprunorum plum death.

Rods. Motile with a polar flagellum. Gram-positive (1931). Gram-negative (1932).

Gelatin: Liquefied.

Agar colonies: White.

Broth plus 5 per cent sucrose: White and cloudy.

Nitrites not produced from nitrates.

Acid but no gas from glucose, lactose, sucrose and glycerol. Starch not hydrolyzed. Strict aerobe.

Comment: Possibly a green fluorescent organism since it produces a faint yellow color in Uschinsky's solution.

Distinctive characters: Differs from Pseudomonas prunicola (Pseudomonas syringae) in that it produces a white cloudy growth in broth plus 5 per cent sucrose, a rapid acid production in nutrient agar plus 5 per cent sucrose, and a faint yellow or no color in Uschinsky's solution.

Source: Isolated from cankers on plum trees in England.

Habitat: Pathogenic on Prunus spp.

87. Pseudomonas papulans Rose, 1917. (Phytopath., 7, 1917, 198.) pa'pu.lans. L. v. papulo to produce pustules; L. part.adj. papulans producing pustules.

Rods 0.8 by 0.8 to 2.5 microns. Motile with 1 to 6 polar flagella. Gram-negative.

Green, fluorescent pigment produced in various media.

Gelatin: Liquefied.

Broth: Turbid with pellicle.

Fermi's and Uschinsky's solutions: Good growth.

Milk: Litmus reduced; no acid.

Nitrites not produced from nitrates. Nitrites produced from nitrates (Burkholder and Starr, Phytopath., 38, 1948, 498).

Indole not produced.

Hydrogen sulfide not produced.

Acid but no gas from glucose, fructose, galactose, mannose, arabinose, xylose, sucrose, glycerol, mannitol, sorbitol, salicin and esculin.

No acid or gas from rhamnose, lactose, maltose, raffinose, trehalose, melizitose, starch, inulin, dextrin, dulcitol or arbutin.

Alkaline reaction produced in glycogen and in acetic, citric, formic, lactic, malic and succinic acids.

Temperature relations: Optimum, 27° C. Minimum, 3.5° C. Maximum, 34.5° C.

Chemical tolerance: Optimum pH, 7.0. Minimum, 5.0. Maximum, 9.4.

Source: Twenty-five cultures isolated from blisters on apples and from rough bark.

Habitat: Pathogenic on apple trees.

88. Pseudomonas pseudozoogloeae (Honing, 1914) Stapp, 1928. (Bacterium pseudozoogloeae Honing, Bull, van Het. Deli Proefstation, Medan, 1, 1914, 7; Stapp, in Sorauer, Handbuch der Pflanzenkrankheiten, 2, 5 Aufl., 1928, 274.)

pseu.do.zo.o.gloe'ae. Gr. pseudes false; Gr. zoum animal; Gr. gloea glue; M.L. fem.noun Zoogloea bacterial generic name; M.L. fem.gen.noun pseudozoogloeae of a false zoogloea.

Rods 0.7 to 1.5 by 0.9 to 2.5 microns. Chains. Motile with 1 or 2 polar flagella. Gram-negative.

Green fluorescent pigment produced in culture.

Gelatin: Liquefied.

Agar colonies: Round, flat, yellow-gray.

Broth: Moderate turbidity with pseudozoogloeae in the pellicle.

Milk: Coagulation. No clearing.

Nitrites not produced from nitrates.

Indole not produced.