Page:Bergey's manual of determinative bacteriology.djvu/149

 30° and 32° C. Minimum between 8° and 9° C. Maximum, 40° C.

pH range, 4.6 to 9.1.

Source: Repeatedly isolated from diseased gladiolus.

Habitat: Pathogenic on Gladiolus spp. and Iris spp.

74. Pseudomonas medicaginis Sackett, 1910. (Science, 31, 1910, 553; also Colorado Agr. Exp. Sta., Bull. 158, 1910, 11.)

me.di.ca'gi.nis. Gr. mēdice the Median grass, alfalfa, lucerne, medic; M.L. fern, noun Medicago generic name of alfalfa; M.L. fem.gen.noun medicaginis of lucerne or alfalfa.

Rods 0.7 by 1.2 microns. Motile with 1 to 4 flagella. Filaments present. Gram-negative.

Green fluorescent pigment produced in culture.

Gelatin: Not liquefied.

Nutrient agar colonies: Growth in 24 hours whitish, glistening.

Broth: Turbid in 24 hours. Pellicle formed. Viscid sediment.

Milk: Becomes alkaline. No change.

Nitrites not produced from nitrates.

Indole not produced.

Hydrogen sulfide not produced.

Not lipolytic (Starr and Burkholder, Phytopath., 32, 1942, 601).

Starch not hydrolyzed.

No gas from carbohydrates. Acid from sucrose.

Slight growth in broth plus 3.75 per cent salt.

Temperature relations: Optimum between 28° and 30° C. Maximum, 37.5°C.

Aerobic.

Source: Isolated from brown lesions on leaves and stems of alfalfa.

Habitat: Pathogenic on alfalfa, Medicago sp.

75. Pseudomonas phaseolicola (Burkholder, 1926) Dowson, 1943. (Phytomonas medicaginis var. phaseolicola Burkholder, Phytopath., 16, 1926, 915; Dowson, Trans. Brit. Mycol. Soc, 26, 1943, 10.)

pha.se.o.li'co.la. Gr. phaseolus the kidney bean; L. dim.mas.noun phaseolus the kidney bean; L. mas.gen.noun phaseoli of the bean; L. cola dweller; M.L. fem.noun phaseolicola the bean dweller.

Description from Burkholder and Zaleski (Phytopath., 22, 1932, 85).

Rods 1.0 by 2.0 microns, sometimes slightly curved; filaments present. Motile with a polar flagellum. Gram-negative.

Green fluorescent pigment produced in culture.

Gelatin stab: Slow liquefaction.

Beef extract agar: Whitish, circular colonies, 2 mm in diameter. Edges entire.

Broth: Turbid.

Milk: Alkaline.

Nitrites not produced from nitrates.

Indole not produced.

Hydrogen sulfide not produced.

Not lipolytic (Starr and Burkholder, Phytopath., 32, 1942, 601).

Acid but no gas from glucose, fructose, mannose, arabinose, xylose, sucrose and glycerol. No acid from rhamnose, lactose, maltose, mannitol or salicin. Alkali from salts of citric and malic acids, but not from acetic, formic, lactic or tartaric acids. Starch and cellulose not hydrolyzed.

Slight growth in broth plus 4 per cent salt.

Temperature relations: Optimum between 20° and 23° C. Minimum, 2.5° C. Maximum, 33° C. (Hedges, Jour. Agr. Res., 36, 1928, 428).

Chemical tolerance: Optimum pH between 6.7 and 7.3. Minimum between 5.0 and 5.3. Maximum between 8.8 and 9.2 (Kotte, Phyt. Zeitsch., 2, 1930 453).

Microaerophilic.

Source: Isolated from leaves, pod and stem of beans showing halo blight.

Habitat: Pathogenic on beans (Phaseolus vulgaris), the kudzu vine (Pueraria hirsuta) and related plants.

76. Pseudomonas pisi Sackett, 1916. (Colorado Agr. Exp. Sta., Bull. 218, 1916, 19.)

pi'si. Gr. pisus or pisum the pea; M.L. neut.noun Pisum generic name of the pea; M.L. neut.gen.noun pisi of the pea.

Rods 0.68 to 2.26 microns. Motile with a polar flagellum. Gram-negative.

Green fluorescent pigment produced in culture.

Gelatin: Liquefied.