Page:Bergey's manual of determinative bacteriology.djvu/145

 Source: Isolated from diseased lettuce from Louisiana.

Habitat: Pathogenic on lettuce, Lactuca saliva.

64. Pseudonionas delphinii (Smith, 1904) Stapp, 1928. (Bacillus delphinii Smith, Science, 19, 1904, 417; Stapp, in Sorauer, Handbuch der Pflanzenkrankheiten, 2, 5 Aufl., 1928, 106.)

del.phi'ni.i. Gr. delphinium the larkspur; M.L. dim.neut.noun Delphinium generic name; M.L. gen.noun delphinii of larkspur.

Rods 0.6 to 0.8 by 1.5 to 2.0 microns. Chains present. Motile, with 1 to 6 polar flagella. Encapsulated. Gram-negative.

Green fluorescent pigment produced in culture.

Gelatin: Liquefied.

Beef agar slants: Growth thin, smooth, shining, transparent, margins entire, crystals. Agar becomes dark brown.

Broth: Turbid in 24 hours with delicate pellicle.

Milk: Becomes alkaline and clears.

Nitrites produced from nitrates (Burkholder and Starr, Phytopath., 38, 1948, 498).

Indole not produced.

Hydrogen sulfide not produced.

Lipolytic action negative (Starr and Burkholder, Phytopath., 32, 1942, 601).

Acid from glucose, galactose and fructose; slight acid from sucrose. No acid from lactose, maltose, glycerol or mannitol.

Starch: Hydrolysis feeble.

Weak growth in broth plus 4 per cent salt.

Optimum pH, 6.7 to 7.1. pH range, 5.6 to 8.6.

Temperature relations: Optimum, 25° C. Minimum, 1° C. or less. Maximum, 30° C. Source: Isolated from black spot of delphinium.

Habitat: Pathogenic on delphinium causing a black spot in the leaves.

65. Pseudomonas cepacia Burkholder, 1950. (Phytopath., 40, 1950, 116.)

ce.pa'ci.a. L. fem.noun caepa or cepa onion; M.L. adj. cepacius of or like onion.

Rods, 0.8 by 1.0 to 2.8 microns, occurring singly or in pairs. Motile, with 1 to 3 polar flagella. Gram-negative.

Gelatin: Slow liquefaction.

Beef-extract-peptone agar: Slants sulfur-yellow, filiform, butyrous to slightly viscid. Most cultures appear rough. Yellow to yellow-green pigment diffuses into medium about the colony.

Potato dextrose agar: Pale yellow. No change in medium.

Broth: Turbid in 24 hours; yellow pellicle.

Milk: Litmus reduced. Medium clears and becomes tan with a yellow pellicle.

Krumwiede's Triple sugar agar: Growth very abundant, yellow-green and extremely wrinkled; medium becomes red.

Nitrites produced from nitrates.

Indole not produced.

Hydrogen sulfide not produced.

Acid but no gas from glucose, fructose, lactose, maltose, sucrose, arabinose, xylose, glycerol, mannitol and salicin; alkaline reaction from sodium salts of citric, hippuric, malonic and tartaric acids. Growth is slight in rhamnose. 2 per cent ethyl alcohol not utilized.

Starch not hydrolyzed.

Sodium ammonium pectate medium not liquefied.

Methyl red test negative; acetylmethylcarbinol not produced.

Growth in 3 per cent but not in 5 per cent salt.

Temperature relations: Optimum, 30° C. Minimum, between 6° and 9° C. Maximum, 42° C.

Aerobic.

Source: Seven isolates from different onion bulbs collected in New York State. Habitat: Pathogenic on onions, Allium cepa.

66. Pseudomonas apii Jagger, 1921. (Jour. Agr. Res., 21, 1921, 186.) a'pi.i. L. apium celery; M.L. neut.noun Apium generic name of celery; M.L. neut.gen.noun apii of celery.

Description from Clara (Cornell Agr. Exp. Sta. Mem. 159, 1934, 24).

Rods 0.75 to 1.5 by 1.5 to 3.0 microns. Motile with a polar flagellum. Gram-negative.

Green fluorescent pigment produced in various media.

Gelatin: Liquefied.

Beef-extract agar colonies: Circular,