Page:Bergey's manual of determinative bacteriology.djvu/144

 Habitat : Causes the angular leaf spot of tobacco (Nicotiana tabacum).

61. Pseudomonas aptata (Brown and Jamieson, 1913) Stevens, 1925. (Bacterium aptatum Brown and Jamieson, Jour. Agr. Res., 1, 1913, 206; Stevens, Plant Disease Fungi, New York, 1925, 22.)

ap.ta'ta. L. part. adj. aptatus adapted.

Rods 0.6 by 1.2 microns. Motile, with bipolar flagella. Gram-negative.

Green fluorescent pigment produced in culture.

Gelatin: Liquefied.

Agar slants: Moderate growth along streak, filiform, whitish, glistening.

Broth: Turbid, with formation of a pellicle.

Milk: Becomes alkaline and clears.

Nitrites not produced from nitrates.

Indole not produced in 10 days. Slight amount found later.

Hydrogen sulfide not produced.

Acid from glucose, galactose and sucrose. No acid from lactose, maltose or mannitol (Paine and Banfoot, Ann. Appl. Biol., 11, 1924, 312).

Starch not hydrolyzed.

Slight growth in broth plus 7 per cent salt (Burkholder).

Temperature relations: Optimum, between 27° and 28° C. Minimum, below 1° C. Maximum, between 34° and 35° C.

Aerobic.

Source : Isolated from diseased nasturtium leaves from Virginia and diseased beet leaves from Utah.

Habitat: Pathogenic on sugar beets, nasturtiums and lettuce.

62. Pseudomonas primulae (Ark and Gardner, 1936) Starr and Burkholder, 1942. (Phytomonas primulae Ark and Gardner, Phytopath., 26, 1936, 1053; Starr and Burkholder, Phytopath., 32, 1942, 601.)

pri'mu.lae. L. dim. adj. prinmlus the first; M.L. fem.noun Primula generic name; M.L. gen.noun primulae of Primula. Rods 0.51 to 0.73 by 1.0 to 3.16 microns. Motile, with a polar flagellum. Gram-negative.

Green fluorescent pigment produced in culture.

Gelatin: Liquefied.

Agar colonies: Round, convex, smooth, glistening, yellowish.

Milk: Coagulated.

Nitrites not produced from nitrates.

Indole not produced.

Hydrogen sulfide not produced.

Not lipolytic (Starr and Burkholder, Phytopath., 32, 1942, 601).

Acid but no gas from glucose, lactose, sucrose, maltose, galactose, arabinose, glycerol, dulcitol and mannitol. Starch not hydrolyzed.

Growth in broth plus 5 per cent salt.

Temperature relations: Optimum between 19° and 22° C. Minimum, 10° C. Maximum, 34° C.

Optimum pH between 6.8 and 7.0. Minimum, between 4.5 and 5.0.

Aerobic, facultative.

Source : Isolated from leaf-spot of Primula polyantha.

Habitat: Pathogenic on Primula spp.

63. Pseudomonas viridilivida (Brown, 1915) Holland, 1920. (Bacterium viridilividum Brown, Jour. Agr. Res., 4, 1915, 475; Holland, Jour. Bact., 5, 1920, 225.)

vi.ri.di.li'vi.da. L. viridis green; L. lividus blue; M.L. adj. viridilividus greenish blue.

Rods 1.0 to 1.25 by 1.25 to 3.0 microns. Motile, with 1 to 3 polar flagella. Gram-negative.

Green fluorescent pigment produced in culture.

Gelatin: Slow liquefaction.

Beef agar colonies: Cream-white, round, smooth, translucent, edges entire.

Broth: Turbid, becomes lime-green.

Milk: Alkaline and clears.

Nitrites produced from nitrates (Burkholder and Starr, Phytopath., 38, 1948, 498).

Indole produced.

Not lipolytic (Starr and Burkholder, Phytopath., 32, 1942, 601).

Acid from glucose and sucrose (Burkholder).

Grows well in 4.5 per cent salt. Grows in 7 per cent salt (Burkholder).

Temperature relations: Minimum, 1.5° C. Maximum, 34.5° C.

Aerobic.