Page:Bergey's manual of determinative bacteriology.djvu/143

 Salt range: 0.25 to 6.0 percent. Optimum, 1.0 to 4.0 per cent.

Source: Sea water.

Habitat: Common along the coast of the North Pacific.

58. Pseudomonas beijerinckii Hof, 1935. (Travaux botaniques néerlandais, 32, 1935, 152.)

bei.jer.inck'i.i. M.L. gen. noun beijerinckii of Beijerinck; named for Prof. M. W. Beijerinck of Delft, Holland.

Small rods. Motile with polar flagella.

Gelatin: No liquefaction.

Indole not produced.

Nitrites produced from nitrates by four out of six strains.

Cellulose not decomposed.

Acid from glucose. In yeast-water with 2 per cent glucose and 12 per cent NaCl, no gas is produced.

Pigment production: Insoluble purple pigment produced but not in all media; is localized markedly; reduced oxygen tension necessary; optimum pH, 8.0; not produced in yeast-water or in peptone-water; produced only when grown in extracts of beans or some other vegetable.

Aerobic.

Source: Six strains isolated from beans preserved with salt.

Habitat: Causes purple discoloration of salted beans.

59. Pseudomonas aceris (Ark, 1939) Starr and Burkholder, 1942. (Phytomonas aceris Ark, Phytopath., 29, 1939, 969; Starr and Burkholder, Phytopath., 32, 1942, 601.)

a'ce.ris. L. acer the maple; L. neut. gen.noun aceris of the maple.

Rods 0.3 to 0.8 by 0.8 to 2.5 microns. Motile, with 1 to 2 polar flagella. Gram-negative.

Green fluorescent pigment produced.

Gelatin: Liquefied.

Beef-extract-peptone agar: Colonies are grayish white, appearing in 24 hours. Broth: Turbid.

Milk: Clearing with no coagulation.

Nitrites produced from nitrates (Burkholder and Starr, Phytopath., 38, 1948, 498).

Indole not produced.

Hydrogen sulfide not produced.

Acid from glucose, fructose, galactose, arabinose, xylose, sucrose, maltose, lactose, raffinose, mannitol, glycerol and dulcitol.

Slight growth in broth plus 6 per cent salt (Burkholder).

Optimum temperature, between 13° and 31° C.

Source: From diseased leaves of the large leaf maple, Acer macrophyllum.

Habitat: Causes a disease of Acer spp.

60. Pseudomonas angulata (Fromme and Murray, 1919) Holland, 1920. (Bacterium angulatum Fromme and Murray, Jour. Agr. Res., 16, 1919, 219; Holland, Jour. Bact., 5, 1920, 224.) ang.u.la'ta. L. part.adj. angulatus with angles, angular.

Description from Clara (Cornell Agr. Exp. Sta. Mem. 159, 1934, 24).

Rods 0.75 to 1.5 by 1.5 to 3.0 microns. Motile, with 1 to 6 polar flagella. Gram-negative.

Gelatin: Liquefied.

Green fluorescent pigment produced.

Beef-extract agar colonies: Dull white, circular, raised, smooth and glistening.

Broth: Turbid and greenish in 36 hours.

Milk: Alkaline.

Nitrites produced from nitrates (Burkholder and Starr, Phytopath., 38, 1948, 498).

Indole not produced.

Hydrogen sulfide not produced.

Lipolytic action negative (Starr and Burkholder, Phytopath., 32, 1942, 601).

Acid but no gas from glucose, galactose, fructose, mannose, arabinose, xylose, sucrose and mannitol. Alkaline reaction from salts of citric, malic, succinic and tartaric acids. Rhamnose, maltose, lactose, raffinose, glycerol, salicin, and acetic, lactic and formic acids are not fermented.

Starch not hydrolyzed.

Slight growth in broth plus 5 to 6 per cent salt (Burkholder).

Aerobic, facultative.

Relationship to other species: Braun (Phytopath., 27, 1937, 283) considers this species to be identical in culture with Pseudomonas tabaci, but they differ in the type of disease they produce.

Source: Isolated by Fromme and Murray from small angular leaf spots on tobacco.