Page:Bergey's manual of determinative bacteriology.djvu/141

 lophotrichous flagella. Encapsulated. Gram-negative.

Gelatin stab : Growth filiform, best at top, 1 with slow crateriform liquefaction.

Agar colonies: Circular, 1.0 to 2.5 mm, with crinkled surface.

Agar slant: Moderate, beaded, raised growth. Membranous consistency. Becomes browned with age.

Broth: Slight turbidity, flocculent sediment, film of growth on walls of test tube.

Milk: No growth.

Potato: No growth.

Indole not produced.

Nitrites not produced from nitrates.

Hydrogen sulfide not produced.

Acid but no gas from glucose, sucrose, dextrin and mannitol. No acid from lactose or xylose.

Starch not hydrolyzed.

Optimum temperature, between 20° and 25° C.

Aerobic.

Source: Isolated from sea water.

Habitat: Sea water.

54. Pseudomonas gelatica (Gran, 1902) Bergey et al., 1930. (Bacillus gelaticus Gran, Bergens Museums Aarbog., 1902, 14; Bergey et al.. Manual, 3rd ed., 1930, 175.)

ge.la'ti.ca. L. part. adj. gelatus frozen, congealed, jellied; M.L. adj. gelaticus resembling hardened gelatin.

Rods, with rounded ends, 0.6 to 1.2 by 1.2 to 2.6 microns, occurring singly, in pairs, and sometimes in short chains. Motile, type of flagellation not recorded. Gram-negative.

All media prepared with 3 per cent salt.

Fish-gelatin colonies: Circular, transparent, glistening, becoming brownish in color.

Fish-gelatin stab: Liquefaction infundibuliform. Two varieties are recognized: one produces a green fluorescence; the other does not produce a water-soluble pigment.

Sea-weed agar colonies: Circular, flat, entire, glistening, grayish blue center with reddish brown periphery. Liquefied.

Fish-agar slant: Flat, transparent streak, with undulate margin, reddish brown to grayish white.

Broth: Turbid with flocculent pellicle and grayish yellow sediment, viscid.

Indole not produced.

Nitrites not produced from nitrates.

Starch hydrolyzed.

No action on sugars.

Aerobic, facultative.

Temperature relations : Optimum temperature, between 20° and 25° C. Maximum, between 30° and 32° C. Minimum, 0° C.

Distinctive character: Requires 3 to 4 per cent salt for growth.

Source : Isolated from sea water from the Norwegian coast.

Habitat: Probably associated with the decomposition of algae in coastal waters.

55. Pseudomonas calcis (Drew, 1912) Kellerman and Smith, 1914. (Bacterium calcis Drew, Yearbook Carnegie Inst. Wash., 11, 1912, 136; Kellerman and Smith, Jour. Wash. Acad. Sci., 4, 1914, 400.)

cal'cis. L. fem.noun calx, calcis limestone, chalk; L. calcis of limestone.

Rods, 1.1 by 1.5 to 3.0 microns, usually single but may form long chains. Actively motile with one polar flagellum. Gram-negative.

Grows best in sea water or 3 per cent salt media. Deposits CaCO3.

Agar colonies: Circular, with finely irregular outline, granular appearance, elevated, spreading; old colonies having brownish tinge in center.

Gelatin stab: Infundibuliform liquefaction.

Gelatin colonies : Small, with liquefaction.

Broth: Good growth especially in presence of potassium nitrate, peptone or calcium malate.

Acid from glucose, mannitol and sucrose but not from lactose.

Nitrates reduced to nitrites and ammonia.

Aerobic, facultative.

Optimum temperature, between 20° and 28° C.

Habitat: Sea water and marine mud.

56. Pseudomonas calciprecipitans Molisch, 1925. (Cent. f. Bakt., II Abt., 65, 1925, 130.)

cal.ci.pre.ci'pi.tans. L. calx, calcis lime; L. praecipito to throw down; M.L. part.adj. calciprecipitans lime-precipitating.