Page:Bergey's manual of determinative bacteriology.djvu/139

 Source: Isolated from soil.

Habitat: Soil.

48. Pseudomonas indoloxidans Gray, 1928. (Proc. Roy. Soc. London, B, 102, 1928, 263.)

in.dol.o′xi.dans. M.L. neut. n. indolum indole; M.L. part. adj. oxidans oxidizing; from Gr. adj. oxys sharp, acid; M.L. part, adj. indoloxidans indole-oxidizing.

Rods 1.0 by 3.0 microns. Motile with one to four polar flagella. Gram-negative

Gelatin colonies: Round, convex, buff, smooth, glistening, erose.

Gelatin stab: No liquefaction.

Agar colonies: Round, convex, white, watery; transparent border, erose.

Agar slant: Filiform, convex, whitish, smooth, glistening, undulate.

Broth: Cloudy.

Indole not produced.

Nitrites produced from nitrates. No gas.

No acid or gas from glucose, sucrose, lactose, maltose or glycerol.

Starch not hydrolyzed.

Phenol and m-cresol not attacked.

Distinctive character: Indole decomposed in mineral salts agar medium with the formation of blue crystals of indigotin.

Aerobic.

Optimum temperature, between 25° and 28° C.

Source: Isolated from soil from Italian Tyrol.

Habitat: Soil.

49. Pseudomonas mira McBeth, 1916. (Soil Science, 1, 1916, 467.)

mi′ra. L. adj. mirus extraordinary.

Rods 0.4 by 1.6 microns. Motile with a single polar flagellum. Gram-negative.

Gelatin stab: Good growth. No liquefaction.

Agar colonies: Circular, convex, grayish white, granular, lacerate.

Agar slant: Moderate, flat, grayish white, somewhat iridescent.

Broth: Turbid.

Litmus milk: Alkaline.

Potato: Moderate, grayish white, leathery growth.

Indole not produced.

Nitrites produced from nitrates.

Ammonia is produced.

No acid from glucose, maltose, lactose, sucrose, starch, glycerol or mannitol.

Cellulose decomposed. Filter paper strips disintegrated at surface of liquid medium.

Aerobic, facultative.

Optimum temperature, 20° C.

Source: Isolated from soil.

Habitat: Soil.

50. Pseudomonas nigrifaciens White, 1940. (Scientific Agriculture, 20, 1940, 643.)

ni.gri.fa′ci.ens. L. niger black; L. v. facio to make; M.L. part. adj. nigrofaciens blackening.

Rods, 0.5 by 1.5 to 2.0 microns, occurring singly or in pairs and having rounded ends. Actively motile with a single polar flagellum. Gram-negative.

Gelatin stab: Pigmented surface growth after 24 hours. Slight crateriform liquefaction changing to saccate.

Agar colonies: Circular, convex, smooth, glistening, entire, 2 to 4 mm in diameter. Slight fluorescence in early stages. The medium assumes a brownish color.

Agar slant: Growth filiform, smooth, moist, glistening, with blackish pigmentation at 4° and 15° C. in 48 hours, the medium turning brownish. Slight fluorescence in early stages.

Broth: Turbid after 24 hours. After 5 to 6 days a black ring and then a pellicle forms, later a black sediment. Medium turns brown.

Litmus milk: A black ring appears after 3 days at 15° C. followed by a pellicle. Litmus is reduced. Alkaline reaction. No coagulation. Digested with a putrid odor.

Potato: No growth, even in presence of 1.5 per cent salt.

Nitrites not produced from nitrates in 7 days. No gas produced.

Starch is hydrolyzed. Natural fats not hydrolyzed.

Alkaline reaction produced in sucrose, maltose, lactose, glucose, mannitol and raffinose broth (pH 8.2). No gas produced.

Ammonia produced in peptone broth.

Aerobic.

Optimum pH, 6.8 to 8.4.

Temperature relations: Minimum, 4° C. Optimum, 25° C. Maximum, 33° to 35° C.